Phylogenetic signal at the Cytb-SertRNA-IG1-ND1 mitochondrial region in Anopheles (Kerteszia) neivai Howard, Dyar & Knab, 1913

• Published in: Biomédica
• Main Authors: Andrés López-Rubio, Juan David Suaza, Charles Porter, Sandra Uribe, Gabriel Bedoya, Iván Darío Vélez
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Instituto Nacional de Salud 2017
• Subjects: Anopheles; malaria; RNA; transfer; DNA; mitochondrial; Colombia; Panamá; Guatemala; Medicine; R; Arctic medicine. Tropical medicine; RC955-962; Arctic; Dyar
• Online Access: https://doi.org/10.7705/biomedica.v37i0.3452; https://doaj.org/article/7812d5b124814a69945d0a6e81471807

Introduction: Mitochondrial DNA has proven its utility for the study of insect evolution. Genes such as cytochrome b (Cytb) and the transfer gene for serine (SertRNA) can be used to compare closely related organisms. Objective: The phylogenetic utility of Cytb-SertRNA-IG1-ND1 was tested for polymorphisms, and secondary structure modeling in SertRNA was done to detect possible cryptic species in Anopheles neivai. Materials and methods: Specimens from Colombia, Guatemala, and the type locality in Panamá were collected and sequenced for specimen comparison based on DNA polymorphisms, and secondary structure modeling for the SertRNA gene. Results: Thirty-six sequences for A. neivai and A. pholidotus were obtained. Conclusions: Polymorphic variants were detected in A. neivai for Cytb-SertRNA-IG1- ND1. Despite this variation in A. neivai, cryptic species could not be detected.