Phylogenetic signal at the Cytb-SertRNA-IG1-ND1 mitochondrial region in Anopheles (Kerteszia) neivai Howard, Dyar & Knab, 1913

Introduction: Mitochondrial DNA has proven its utility for the study of insect evolution. Genes such as cytochrome b (Cytb) and the transfer gene for serine (SertRNA) can be used to compare closely related organisms. Objective: The phylogenetic utility of Cytb-SertRNA-IG1-ND1 was tested for polymorp...

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Bibliographic Details
Published in:Biomédica
Main Authors: Andrés López-Rubio, Juan David Suaza, Charles Porter, Sandra Uribe, Gabriel Bedoya, Iván Darío Vélez
Format: Article in Journal/Newspaper
Language:English
Spanish
Published: Instituto Nacional de Salud 2017
Subjects:
Anopheles
malaria
RNA
transfer
DNA
mitochondrial
Colombia
Panamá
Guatemala
Medicine
R
Arctic medicine. Tropical medicine
RC955-962
Arctic
Dyar
Online Access:https://doi.org/10.7705/biomedica.v37i0.3452
https://doaj.org/article/7812d5b124814a69945d0a6e81471807
Description
Summary:Introduction: Mitochondrial DNA has proven its utility for the study of insect evolution. Genes such as cytochrome b (Cytb) and the transfer gene for serine (SertRNA) can be used to compare closely related organisms. Objective: The phylogenetic utility of Cytb-SertRNA-IG1-ND1 was tested for polymorphisms, and secondary structure modeling in SertRNA was done to detect possible cryptic species in Anopheles neivai. Materials and methods: Specimens from Colombia, Guatemala, and the type locality in Panamá were collected and sequenced for specimen comparison based on DNA polymorphisms, and secondary structure modeling for the SertRNA gene. Results: Thirty-six sequences for A. neivai and A. pholidotus were obtained. Conclusions: Polymorphic variants were detected in A. neivai for Cytb-SertRNA-IG1- ND1. Despite this variation in A. neivai, cryptic species could not be detected.

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