Genetic survey of caribou populations using microsatellite DNA

Microsatellite loci are highly variable regions of eukaryotic DNA that consist of tandemly repeated sequences of one to six nucleotides in length. The use of microsatellites and the Polymerase Chain Reaction (PCR) are powerful tools for quantifying genetic variation within and among individual popul...

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Bibliographic Details
Published in:Rangifer
Main Authors: James E.E. Kushny, John W. Coffin, Curtis Strobeck
Format: Article in Journal/Newspaper
Language:English
Published: Septentrio Academic Publishing 1996
Subjects:
caribou
microsatellites
DNA fingerprinting
population level analysis
Rangifer tarandus
Animal culture
SF1-1100
Canada
Peary
Rangifer
Online Access:https://doi.org/10.7557/2.16.4.1277
https://doaj.org/article/77ad8d5e31cb46eab119a6a5d035b9dc
Description
Summary:Microsatellite loci are highly variable regions of eukaryotic DNA that consist of tandemly repeated sequences of one to six nucleotides in length. The use of microsatellites and the Polymerase Chain Reaction (PCR) are powerful tools for quantifying genetic variation within and among individual populations. Recently, we have developed primers for caribou that amplify 4 microsatellite loci. These microsatellite loci were used to survey the genetic variation in populations of Barren-ground caribou (Rangifer tarandus groenlandicus), Peary caribou (R.t. pearyi) and Woodland caribou (R.t. caribou) of Canada. The four loci examined were all polymorphic, revealing high levels of heterozygosity (> 0.74) in all of the study populations.

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