Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies Microteste de inibição de fluorescência simplificado para titulação de anticorpos antirábicos

A simplified fluorescence inhibition microtest (SFIMT) was standardized for the evaluation of antirabies serum neutralizing antibodies based on the rapid fluorescent focus inhibition test (RFFIT) and the fluorescence inhibition microtest (FIMT). The simplified test showed reproductibility similar to...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: S.R. Favoretto, M.L. Carrieri, M.S. Tino, C.R. Zanetti, O.A.C. Pereira
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 1993
Subjects:
Simplified Fluorescence Inhibition Microtest
rabies antibodies
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/S0036-46651993000200009
https://doaj.org/article/7553db33faa54f118af1c4a615cbf728
Description
Summary:A simplified fluorescence inhibition microtest (SFIMT) was standardized for the evaluation of antirabies serum neutralizing antibodies based on the rapid fluorescent focus inhibition test (RFFIT) and the fluorescence inhibition microtest (FIMT). The simplified test showed reproductibility similar to that of the FIMT with advantages as easier executation and quicker reading. A simple pre-treatment of Brazilian microplates produced for immune enzymatic assays (PROSIL) gave equivalent results and substantial coast reduction, in relation to imported plates (DIFCO). The simplified test can be easily implemented in less sophisticated laboratories, as alternative to the mouse serum neutralization test, still the most largely employed in Brazil, or even to others as RFFIT and FIMT. No presente trabalho foi padronizada uma microtécnica simplificada para a avaliação de anticorpos neutralizantes anti-rábicos com base no RFFIT e no FIMT. Este teste mostrou reprodutibilidade comparável a dos testes originais, sendo de execução mais simples e de leitura mais rápida. Uma microplaca de fabricação nacional para testes imuno-enzimáticos (PROSIL Ind. e Com.) pode ser utilizada com resultados equivalentes aos de microplacas Difco ou similar, com substancial redução de custos, mediante tratamento simples e rápido. O teste estudado pode ser implantado facilmente em laboratórios menos sofisticados substituindo com grandes vantagens a prova de soro-neutralização em camundongos ainda a mais empregada rotineiramente no Brasil.

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