Screening of Lactic Acid Bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) as potential probiotics

The present study aims to isolate, identify, and evaluate potential probiotic, Lactic Acid Bacteria (LAB) from the gut and hepatopancreas of 10 healthy wild giant freshwater prawn, Macrobrachium rosenbergii. The samples were cultivated using a modified De Man, Rogosa and Sharpe (MRS) agar supplement...

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Bibliographic Details
Published in:Aquaculture Reports
Main Authors: Nurshahieda Mohamad, Hidayah Manan, Mhd Sallehhuddin, Najiah Musa, Mhd Ikhwanuddin
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2020
Subjects:
Turbot
Rearing temperature
scFOS
Gut microbiota
Digestive enzyme
Gut histology
Aquaculture. Fisheries. Angling
SH1-691
Online Access:https://doi.org/10.1016/j.aqrep.2020.100523
https://doaj.org/article/74e074ea2f504b09a4e030dea7b1bbd2
Description
Summary:The present study aims to isolate, identify, and evaluate potential probiotic, Lactic Acid Bacteria (LAB) from the gut and hepatopancreas of 10 healthy wild giant freshwater prawn, Macrobrachium rosenbergii. The samples were cultivated using a modified De Man, Rogosa and Sharpe (MRS) agar supplemented with 2% of CaCO3 (w/v). Bacterial colonies with strong clear zone were selected for further tests. From these, 17 isolates of LAB showed non-spore forming, homofermentative characteristics, Gram-positive, rod-shaped, non-motile bacteria and absences of catalase enzymes. The LAB was further identified using API 50 CH kit and 16S rRNA. The isolates LAB also showed tolerance to a variety of environmental conditions like pH, temperature, salt, bile and hemolysis activity in in vitro condition. Based on the results, two isolates LAB were identified as Lactobacillus plantarum and Entercoccus faecalis were suggested as potential probiotic candidates from giant freshwater prawn for Aquaculture industries.

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