Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic analysis

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Yunita Armiyanti, Renam Putra Arifianto, Elisa Nurma Riana, Kartika Senjarini, Widodo Widodo, Loeki Enggar Fitri, Teguh Wahju Sardjono
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2016
• Subjects: Saliva; Immunogenic; Proteins; Anopheles; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2016.08.012; https://doaj.org/article/72923a095ee941ce89ae2033176b21bf

Objective: To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools. Methods: The identification of antigenic proteins of Anopheles maculatus salivary gland used these techniques: one-dimensional gel electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis), western blot, and liquid chromatography–mass spectrometry. Results: The proteins that have molecular weight (MW) 43 and 34 kDa were the antigenic protein. Computational bioinformatic analysis by Mascot Server revealed seven novel hypothetical proteins (MW: 43 kDa) and two novel hypothetical proteins (MW: 34 kDa). Further analysis (BLASTP, antigenicity, epitope mapping, and specificity analysis) showed that two novel proteins were identified as apolipoprotein D and cathepsin D in Anopheles darlingi. Conclusions: The identified proteins are potential to be developed as a biomarker of mosquito bite's exposure.