Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010

Dengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purp...

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Bibliographic Details
Published in:Journal of Tropical Medicine
Main Authors: Arindam Sarkar, Debjani Taraphdar, Shyamalendu Chatterjee
Format: Article in Journal/Newspaper
Language:English
Published: Hindawi Limited 2012
Subjects:
Online Access:https://doi.org/10.1155/2012/960329
https://doaj.org/article/6f3904b7cc1e4194af642c9eac1845d2
Description
Summary:Dengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purpose. All the samples were tested for the detection of IgM antibodies by ELISA method, followed by RT-PCR test for the detection of serotypes. Only 173 samples were ELISA positive. Out of 378 samples, 108 were RT-PCR positive. Out of 108 samples, 74 samples had monotypic infection with different serotypes of DENV and 33 samples had dual infections with DENV-2 and DENV-3. Only one sample had the infection with DENV-1, DENV-2, and DENV-3. DHF was found mainly among the patients, infected with multiple dengue serotypes. Only 3 dengue monotypic infected patients had suffered from DHF.