Extracellular cold active lipase from the psychrotrophic Halomonas sp. BRI 8 isolated from the Antarctic sea water
An extracellular cold active lipase-producing psychrotrophic bacterium (BRI 8) was isolated from the Antarctic sea water sample. The 16s rRNA sequence study revealed that the isolate belongs to the genus Halomonas (929 bp). The present paper reports optimization of fermentation conditions for produc...
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Prince of Songkla University
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ftdoajarticles:oai:doaj.org/article:6a22c13405dd42aa9028d664dda8f1c2 2023-05-15T13:34:55+02:00 Extracellular cold active lipase from the psychrotrophic Halomonas sp. BRI 8 isolated from the Antarctic sea water Vipra Vijay Jadhav Swanandi Suresh Pote Amit Yadav Yogesh S Shouche Rama Kaustubh Bhadekar 2013-12-01T00:00:00Z https://doaj.org/article/6a22c13405dd42aa9028d664dda8f1c2 EN eng Prince of Songkla University http://rdo.psu.ac.th/sjstweb/journal/35-6/35-6-2.pdf https://doaj.org/toc/0125-3395 0125-3395 https://doaj.org/article/6a22c13405dd42aa9028d664dda8f1c2 Songklanakarin Journal of Science and Technology (SJST), Vol 35, Iss 6, Pp 623-630 (2013) Halomonas Antarctic cold active lipase optimization stability Technology T Technology (General) T1-995 Science Q Science (General) Q1-390 article 2013 ftdoajarticles 2022-12-31T04:30:46Z An extracellular cold active lipase-producing psychrotrophic bacterium (BRI 8) was isolated from the Antarctic sea water sample. The 16s rRNA sequence study revealed that the isolate belongs to the genus Halomonas (929 bp). The present paper reports optimization of fermentation conditions for production of lipase (EC 3.1.1.3) from Halomonas sp. BRI 8. Highest lipase production was observed in the medium containing olive oil and peptone. The optimum pH and temperature for enzyme catalysis were 7.0 and 10°C respectively. The enzyme was relatively more stable in acidic pH range and retained 50% activity when incubated at 30°C for 1 h. The enzyme was stable in various organic solvents and showed more than 100% activity in presence of isoamyl alcohol. Significant enzyme activity was also observed in the presence of metal ions and detergents. The molecular mass of partially purified lipase was found to be around 66 kD. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Halomonas Antarctic cold active lipase optimization stability Technology T Technology (General) T1-995 Science Q Science (General) Q1-390 |
spellingShingle |
Halomonas Antarctic cold active lipase optimization stability Technology T Technology (General) T1-995 Science Q Science (General) Q1-390 Vipra Vijay Jadhav Swanandi Suresh Pote Amit Yadav Yogesh S Shouche Rama Kaustubh Bhadekar Extracellular cold active lipase from the psychrotrophic Halomonas sp. BRI 8 isolated from the Antarctic sea water |
topic_facet |
Halomonas Antarctic cold active lipase optimization stability Technology T Technology (General) T1-995 Science Q Science (General) Q1-390 |
description |
An extracellular cold active lipase-producing psychrotrophic bacterium (BRI 8) was isolated from the Antarctic sea water sample. The 16s rRNA sequence study revealed that the isolate belongs to the genus Halomonas (929 bp). The present paper reports optimization of fermentation conditions for production of lipase (EC 3.1.1.3) from Halomonas sp. BRI 8. Highest lipase production was observed in the medium containing olive oil and peptone. The optimum pH and temperature for enzyme catalysis were 7.0 and 10°C respectively. The enzyme was relatively more stable in acidic pH range and retained 50% activity when incubated at 30°C for 1 h. The enzyme was stable in various organic solvents and showed more than 100% activity in presence of isoamyl alcohol. Significant enzyme activity was also observed in the presence of metal ions and detergents. The molecular mass of partially purified lipase was found to be around 66 kD. |
format |
Article in Journal/Newspaper |
author |
Vipra Vijay Jadhav Swanandi Suresh Pote Amit Yadav Yogesh S Shouche Rama Kaustubh Bhadekar |
author_facet |
Vipra Vijay Jadhav Swanandi Suresh Pote Amit Yadav Yogesh S Shouche Rama Kaustubh Bhadekar |
author_sort |
Vipra Vijay Jadhav |
title |
Extracellular cold active lipase from the psychrotrophic Halomonas sp. BRI 8 isolated from the Antarctic sea water |
title_short |
Extracellular cold active lipase from the psychrotrophic Halomonas sp. BRI 8 isolated from the Antarctic sea water |
title_full |
Extracellular cold active lipase from the psychrotrophic Halomonas sp. BRI 8 isolated from the Antarctic sea water |
title_fullStr |
Extracellular cold active lipase from the psychrotrophic Halomonas sp. BRI 8 isolated from the Antarctic sea water |
title_full_unstemmed |
Extracellular cold active lipase from the psychrotrophic Halomonas sp. BRI 8 isolated from the Antarctic sea water |
title_sort |
extracellular cold active lipase from the psychrotrophic halomonas sp. bri 8 isolated from the antarctic sea water |
publisher |
Prince of Songkla University |
publishDate |
2013 |
url |
https://doaj.org/article/6a22c13405dd42aa9028d664dda8f1c2 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Songklanakarin Journal of Science and Technology (SJST), Vol 35, Iss 6, Pp 623-630 (2013) |
op_relation |
http://rdo.psu.ac.th/sjstweb/journal/35-6/35-6-2.pdf https://doaj.org/toc/0125-3395 0125-3395 https://doaj.org/article/6a22c13405dd42aa9028d664dda8f1c2 |
_version_ |
1766059044992188416 |