Extracellular cold active lipase from the psychrotrophic Halomonas sp. BRI 8 isolated from the Antarctic sea water

An extracellular cold active lipase-producing psychrotrophic bacterium (BRI 8) was isolated from the Antarctic sea water sample. The 16s rRNA sequence study revealed that the isolate belongs to the genus Halomonas (929 bp). The present paper reports optimization of fermentation conditions for produc...

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Bibliographic Details
Main Authors: Vipra Vijay Jadhav, Swanandi Suresh Pote, Amit Yadav, Yogesh S Shouche, Rama Kaustubh Bhadekar
Format: Article in Journal/Newspaper
Language:English
Published: Prince of Songkla University 2013
Subjects:
Halomonas
Antarctic
cold active lipase
optimization
stability
Technology
T
Technology (General)
T1-995
Science
Q
Science (General)
Q1-390
The Antarctic
Antarc*
Online Access:https://doaj.org/article/6a22c13405dd42aa9028d664dda8f1c2
Description
Summary:An extracellular cold active lipase-producing psychrotrophic bacterium (BRI 8) was isolated from the Antarctic sea water sample. The 16s rRNA sequence study revealed that the isolate belongs to the genus Halomonas (929 bp). The present paper reports optimization of fermentation conditions for production of lipase (EC 3.1.1.3) from Halomonas sp. BRI 8. Highest lipase production was observed in the medium containing olive oil and peptone. The optimum pH and temperature for enzyme catalysis were 7.0 and 10°C respectively. The enzyme was relatively more stable in acidic pH range and retained 50% activity when incubated at 30°C for 1 h. The enzyme was stable in various organic solvents and showed more than 100% activity in presence of isoamyl alcohol. Significant enzyme activity was also observed in the presence of metal ions and detergents. The molecular mass of partially purified lipase was found to be around 66 kD.

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