Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf ( Canis lupus )
The discovery of novel probiotic bacteria from free-ranging animals for the treatment of inflammatory bowel disease in domestic pets is a unique approach. The chloroform extraction of gastrointestinal (GI) tract material was used to inactivate vegetative cells and select for spore-forming bacteria....
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2023
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Online Access: | https://doi.org/10.3390/applmicrobiol3040077 https://doaj.org/article/66c9cb5c14a544e7ad51c59cbc79778b |
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ftdoajarticles:oai:doaj.org/article:66c9cb5c14a544e7ad51c59cbc79778b 2024-01-28T10:05:03+01:00 Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf ( Canis lupus ) Jennifer McCabe Jessika L. Bryant C. Cristoph Klews MiCayla Johnson Ariel N. Atchley Thomas W. Cousins Analiska Dominguez Marie Gabriel Katie Middleton Natasha A. Bowles Heather M. Broughton Kristina M. Smith Mark R. Ackermann Robert Bildfell Patrick N. Ball Evan S. Forsythe Bruce S. Seal 2023-09-01T00:00:00Z https://doi.org/10.3390/applmicrobiol3040077 https://doaj.org/article/66c9cb5c14a544e7ad51c59cbc79778b EN eng MDPI AG https://www.mdpi.com/2673-8007/3/4/77 https://doaj.org/toc/2673-8007 doi:10.3390/applmicrobiol3040077 2673-8007 https://doaj.org/article/66c9cb5c14a544e7ad51c59cbc79778b Applied Microbiology, Vol 3, Iss 4, Pp 1120-1129 (2023) probiotic canine inflammatory bowel disease antimicrobial genomics Paenibacillaceae Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.3390/applmicrobiol3040077 2023-12-31T01:39:22Z The discovery of novel probiotic bacteria from free-ranging animals for the treatment of inflammatory bowel disease in domestic pets is a unique approach. The chloroform extraction of gastrointestinal (GI) tract material was used to inactivate vegetative cells and select for spore-forming bacteria. A bacterium identified as a novel Paenibacillus sp. strain via small ribosomal RNA (16S) gene sequencing was isolated from the GI tract of a gray wolf ( Canis lupus ). The bacterium was typed as Gram-variable, both catalase/oxidase-positive and positive via starch hydrolysis and lipase assays. The bacterium inhibited the growth of Staphylococcus aureus, Escherichia coli and Micrococcus luteus . The draft whole genome sequence (WGS) assembly was 7,034,206 bp in length, encoding 6543 genes, and is similar in size and coding capacity to other closely related Paenibacillus spp. The isolate’s genome encodes several germination and sporulation gene products along with antimicrobials such as a bacteriocin system and chitinase. Enzyme genes such as alpha amylase, cellulase, lipases and pectin lyase are also present in the genome. An incomplete lysogenic bacteriophage genome was also present in the isolate’s genome. Phenotypic characteristics combined with a WGS genotype analysis indicate that this bacterium, designated Paenibacillus sp. ClWae2A, could be a potential candidate probiotic for domestic dogs. Article in Journal/Newspaper Canis lupus gray wolf Directory of Open Access Journals: DOAJ Articles Applied Microbiology 3 4 1120 1129 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
probiotic canine inflammatory bowel disease antimicrobial genomics Paenibacillaceae Microbiology QR1-502 |
spellingShingle |
probiotic canine inflammatory bowel disease antimicrobial genomics Paenibacillaceae Microbiology QR1-502 Jennifer McCabe Jessika L. Bryant C. Cristoph Klews MiCayla Johnson Ariel N. Atchley Thomas W. Cousins Analiska Dominguez Marie Gabriel Katie Middleton Natasha A. Bowles Heather M. Broughton Kristina M. Smith Mark R. Ackermann Robert Bildfell Patrick N. Ball Evan S. Forsythe Bruce S. Seal Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf ( Canis lupus ) |
topic_facet |
probiotic canine inflammatory bowel disease antimicrobial genomics Paenibacillaceae Microbiology QR1-502 |
description |
The discovery of novel probiotic bacteria from free-ranging animals for the treatment of inflammatory bowel disease in domestic pets is a unique approach. The chloroform extraction of gastrointestinal (GI) tract material was used to inactivate vegetative cells and select for spore-forming bacteria. A bacterium identified as a novel Paenibacillus sp. strain via small ribosomal RNA (16S) gene sequencing was isolated from the GI tract of a gray wolf ( Canis lupus ). The bacterium was typed as Gram-variable, both catalase/oxidase-positive and positive via starch hydrolysis and lipase assays. The bacterium inhibited the growth of Staphylococcus aureus, Escherichia coli and Micrococcus luteus . The draft whole genome sequence (WGS) assembly was 7,034,206 bp in length, encoding 6543 genes, and is similar in size and coding capacity to other closely related Paenibacillus spp. The isolate’s genome encodes several germination and sporulation gene products along with antimicrobials such as a bacteriocin system and chitinase. Enzyme genes such as alpha amylase, cellulase, lipases and pectin lyase are also present in the genome. An incomplete lysogenic bacteriophage genome was also present in the isolate’s genome. Phenotypic characteristics combined with a WGS genotype analysis indicate that this bacterium, designated Paenibacillus sp. ClWae2A, could be a potential candidate probiotic for domestic dogs. |
format |
Article in Journal/Newspaper |
author |
Jennifer McCabe Jessika L. Bryant C. Cristoph Klews MiCayla Johnson Ariel N. Atchley Thomas W. Cousins Analiska Dominguez Marie Gabriel Katie Middleton Natasha A. Bowles Heather M. Broughton Kristina M. Smith Mark R. Ackermann Robert Bildfell Patrick N. Ball Evan S. Forsythe Bruce S. Seal |
author_facet |
Jennifer McCabe Jessika L. Bryant C. Cristoph Klews MiCayla Johnson Ariel N. Atchley Thomas W. Cousins Analiska Dominguez Marie Gabriel Katie Middleton Natasha A. Bowles Heather M. Broughton Kristina M. Smith Mark R. Ackermann Robert Bildfell Patrick N. Ball Evan S. Forsythe Bruce S. Seal |
author_sort |
Jennifer McCabe |
title |
Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf ( Canis lupus ) |
title_short |
Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf ( Canis lupus ) |
title_full |
Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf ( Canis lupus ) |
title_fullStr |
Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf ( Canis lupus ) |
title_full_unstemmed |
Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf ( Canis lupus ) |
title_sort |
phenotypic and draft genome sequence analyses of a paenibacillus sp. isolated from the gastrointestinal tract of a north american gray wolf ( canis lupus ) |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/applmicrobiol3040077 https://doaj.org/article/66c9cb5c14a544e7ad51c59cbc79778b |
genre |
Canis lupus gray wolf |
genre_facet |
Canis lupus gray wolf |
op_source |
Applied Microbiology, Vol 3, Iss 4, Pp 1120-1129 (2023) |
op_relation |
https://www.mdpi.com/2673-8007/3/4/77 https://doaj.org/toc/2673-8007 doi:10.3390/applmicrobiol3040077 2673-8007 https://doaj.org/article/66c9cb5c14a544e7ad51c59cbc79778b |
op_doi |
https://doi.org/10.3390/applmicrobiol3040077 |
container_title |
Applied Microbiology |
container_volume |
3 |
container_issue |
4 |
container_start_page |
1120 |
op_container_end_page |
1129 |
_version_ |
1789331105429585920 |