Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf ( Canis lupus )

The discovery of novel probiotic bacteria from free-ranging animals for the treatment of inflammatory bowel disease in domestic pets is a unique approach. The chloroform extraction of gastrointestinal (GI) tract material was used to inactivate vegetative cells and select for spore-forming bacteria....

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Bibliographic Details
Published in:Applied Microbiology
Main Authors: Jennifer McCabe, Jessika L. Bryant, C. Cristoph Klews, MiCayla Johnson, Ariel N. Atchley, Thomas W. Cousins, Analiska Dominguez, Marie Gabriel, Katie Middleton, Natasha A. Bowles, Heather M. Broughton, Kristina M. Smith, Mark R. Ackermann, Robert Bildfell, Patrick N. Ball, Evan S. Forsythe, Bruce S. Seal
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2023
Subjects:
probiotic
canine inflammatory bowel disease
antimicrobial
genomics
Paenibacillaceae
Microbiology
QR1-502
Canis lupus
gray wolf
Online Access:https://doi.org/10.3390/applmicrobiol3040077
https://doaj.org/article/66c9cb5c14a544e7ad51c59cbc79778b
Description
Summary:The discovery of novel probiotic bacteria from free-ranging animals for the treatment of inflammatory bowel disease in domestic pets is a unique approach. The chloroform extraction of gastrointestinal (GI) tract material was used to inactivate vegetative cells and select for spore-forming bacteria. A bacterium identified as a novel Paenibacillus sp. strain via small ribosomal RNA (16S) gene sequencing was isolated from the GI tract of a gray wolf ( Canis lupus ). The bacterium was typed as Gram-variable, both catalase/oxidase-positive and positive via starch hydrolysis and lipase assays. The bacterium inhibited the growth of Staphylococcus aureus, Escherichia coli and Micrococcus luteus . The draft whole genome sequence (WGS) assembly was 7,034,206 bp in length, encoding 6543 genes, and is similar in size and coding capacity to other closely related Paenibacillus spp. The isolate’s genome encodes several germination and sporulation gene products along with antimicrobials such as a bacteriocin system and chitinase. Enzyme genes such as alpha amylase, cellulase, lipases and pectin lyase are also present in the genome. An incomplete lysogenic bacteriophage genome was also present in the isolate’s genome. Phenotypic characteristics combined with a WGS genotype analysis indicate that this bacterium, designated Paenibacillus sp. ClWae2A, could be a potential candidate probiotic for domestic dogs.

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