Phenotypic Characterization and Draft Genome Sequence Analyses of Two Novel Endospore-Forming Sporosarcina spp. Isolated from Canada Goose ( Branta canadensis ) Feces

In an attempt to isolate new probiotic bacteria, two Gram-variable, spore-forming, rod-shaped aerobic bacteria designated as strain A4 and A15 were isolated from the feces of Canada geese ( Branta canadensis ). Strain A4 was able to grow in high salt levels and exhibited lipase activity, while A15 d...

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Bibliographic Details
Published in:Microorganisms
Main Authors: Jitendra Keshri, Kristina M. Smith, Molly K. Svendsen, Haley R. Keillor, Madeline L. Moss, Haley J. Jordan, Abigail M. Larkin, Johnna K. Garrish, John Eric Line, Patrick N. Ball, Brian B. Oakley, Bruce S. Seal
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2023
Subjects:
microbiome
spore-forming bacteria
urease
probiotic
Firmicutes
Planococcaceae
Biology (General)
QH301-705.5
Canada
Branta canadensis
Canada Goose
Online Access:https://doi.org/10.3390/microorganisms12010070
https://doaj.org/article/664fde2cab1243f9ba3426db8fdbf31d
Description
Summary:In an attempt to isolate new probiotic bacteria, two Gram-variable, spore-forming, rod-shaped aerobic bacteria designated as strain A4 and A15 were isolated from the feces of Canada geese ( Branta canadensis ). Strain A4 was able to grow in high salt levels and exhibited lipase activity, while A15 did not propagate under these conditions. Both were positive for starch hydrolysis, and they inhibited the growth of Staphylococcus aureus . The strains of the 16S rRNA sequence shared only 94% similarity to previously identified Sporosarcina spp. The ANI (78.08%) and AAI (82.35%) between the two strains were less than the species threshold. Searches for the most similar genomes using the Mash/Minhash algorithm showed the nearest genome to strain A4 and A15 as Sporosarcina sp. P13 (distance of 21%) and S. newyorkensis (distance of 17%), respectively. Sporosarcina spp. strains A4 and A15 contain urease genes, and a fibronectin-binding protein gene indicates that these bacteria may bind to eukaryotic cells in host gastrointestinal tracts. Phenotypic and phylogenetic data, along with low dDDH, ANI, and AAI values for strains A4 and A15, indicate these bacteria are two novel isolates of the Sporosarcina genus: Sporosarcina sp. A4 sp. nov., type strain as Sporosarcina cascadiensis and Sporosarcina sp. A15 sp. nov., type strain Sporosarcina obsidiansis .

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