Characterization of Leishmania (L.) infantum chagasi in visceral leishmaniasis associated with hiv co-infection in Northeastern Brazil

ABSTRACT Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNe...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Elaine Cristina Bomfim de Lima, Walter Lins Barbosa Júnior, Maria Edileuza Felinto de Brito, Fábio Lopes de Melo, Sinval Pinto Brandão Filho, Zulma Maria de Medeiros
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 2017
Subjects:
Characterization
Leishmania (L.) infantum chagasi
Visceral leishmaniasis
HIV/AIDS
Coinfection
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/s1678-9946201759048
https://doaj.org/article/646688e6c14a4b32a81809c0db1d17cc
Description
Summary:ABSTRACT Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicolle culture and kDNA PCR of peripheral blood were all positive. Typing methods, multilocus enzyme electrophoresis and ITS1-RFLP PCR of peripheral blood confirmed infection by Leishmania (L.) infantum chagasi . PCR has proved to be safer and more affordable than other characterization methods; ITS1-RFLP PCR can diagnose and type Leishmania spp. in both endemic and non-endemic areas, favoring the prognosis and allowing the appropriate treatment of patients.

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