Is qPCR always the most sensitive method for malaria diagnostic quality surveillance?

Abstract In many studies to evaluate the quality of malaria diagnosis, microscopy or rapid diagnostic tests (RDT) are compared to PCR. Depending on the method for sample collection and storage (whole blood or dried blood spot), volume of blood used for extraction, volume of DNA used as PCR template,...

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Bibliographic Details
Published in:Malaria Journal
Main Author: Cristian Koepfli
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2023
Subjects:
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1186/s12936-023-04822-w
https://doaj.org/article/644b46ffe08445319fc584956d8c3b91
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Summary:Abstract In many studies to evaluate the quality of malaria diagnosis, microscopy or rapid diagnostic tests (RDT) are compared to PCR. Depending on the method for sample collection and storage (whole blood or dried blood spot), volume of blood used for extraction, volume of DNA used as PCR template, and choice of PCR target (single vs. multi-copy gene), the limit of detection (LOD) of PCR might not exceed the LOD of expert microscopy or RDT. One should not assume that PCR always detects the highest number of infections.

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