Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L.
Objective: To investigate potential antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. in different in vivo and in vitro experimental models. Methods: In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the pl...
| Published in: | Asian Pacific Journal of Tropical Biomedicine |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2014
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| Subjects: | |
| Online Access: | https://doi.org/10.12980/APJTB.4.2014C1298 https://doaj.org/article/63cd19082e77492d87927413caebbd5d |
| Summary: | Objective: To investigate potential antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. in different in vivo and in vitro experimental models. Methods: In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the plant extract. In vivo analgesic activity was carried out by acetic acid-induced writhing test in Swiss albino mice. All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight. Antibacterial activity was studied by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains. Brine shrimp lethality assay was used to investigate cytotoxicity effects of the plant extract. Results: The extract showed free radical scavenging activity in the DPPH assay (IC50∼41 μg/mL) compared to the standard antioxidant ascorbic acid (IC50∼19 μg/mL). The extract also produced prominent antimicrobial activity against Salmonella typhi, Salmonella paratyphi, Shigella boydii, Streptococcus pyogenes and Streptococcus aureus compared to standard drug kanamycin at the dose of 30 μg/disc. The extract exhibited lethality against the brine shrimp nauplii with the LC50 values of 40 μg/mL, and also 90% mortality (LC90) value was found to be 160 μg/mL. In analgesic test, the extract demonstrated statistically significant (P<0.01) analgesic effect in acetic acid induced writhing in white albino mice at both dose levels. Conclusions: These results suggest that the ethanolic extract of Mentha arvensis L. has potential antioxidant, antibacterial, cytotoxic and analgesic activities that support the ethnopharmacological uses of this plant. |
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