A novel HPTLC method for quantitative estimation of biomarkers in polyherbal formulation

Objective: To explore the quantitative estimation of biomarkers gallic acid and berberine in polyherbal formulation Entoban syrup. Methods: High performance thin layer chromatography was performed to evaluate the presence of gallic acid and berberine employing toluene: ethyl acetate: formic acid: me...

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Bibliographic Details
Published in:Asian Pacific Journal of Tropical Biomedicine
Main Authors: Zeeshan Ahmed Sheikh, Sadia Shakeel, Somia Gul, Aqib Zahoor, Saleha Suleman Khan, Faisal Haider Zaidi, Khan Usmanghani
Format: Article in Journal/Newspaper
Language:English
Published: Wolters Kluwer Medknow Publications 2015
Subjects:
Quantitative estimation
Gallic acid
Berberine
Entoban syrup
HPTLC
Arctic medicine. Tropical medicine
RC955-962
Biology (General)
QH301-705.5
Arctic
Online Access:https://doi.org/10.1016/j.apjtb.2015.06.016
https://doaj.org/article/6046b5ab62c0456daaadf00ffbecd640
Description
Summary:Objective: To explore the quantitative estimation of biomarkers gallic acid and berberine in polyherbal formulation Entoban syrup. Methods: High performance thin layer chromatography was performed to evaluate the presence of gallic acid and berberine employing toluene: ethyl acetate: formic acid: methanol 12:9:4:0.5 (v/v/v/v) and ethanol: water: formic acid 90:9:1 (v/v/v), as a mobile phase respectively. Results: The Rf values (0.58) for gallic acid and (0.76) for berberine in both sample and reference standard were found comparable under UV light at 273 nm and 366 nm respectively. The high performance thin layer chromatography method developed for quantization was simple, accurate and specific. Conclusions: The present standardization provides specific and accurate tool to develop qualifications for identity, transparency and reproducibility of biomarkers in Entoban syrup.

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