Use of Kisser glycerol gelatin to prepare microscope slides of Phlebotomine sandflies (Diptera: Psychodidae)

Abstract INTRODUCTION: Appropriate preservation of specimens is important for taxonomic identification. In sandfly research, various methods have been used for slide preparation; however, high cost, low commercial availability, and associated hazards make their use impossible in some studies. Theref...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical
Main Authors: Vanusa Gonçalves Holanda, Diogo Brunno e Silva Barbosa, Leudimar Aires Pereira, Maria Regiane Araujo Soares
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2019
Subjects:
Sandfly
Taxonomy
Kisser glycerol gelatin
Arctic medicine. Tropical medicine
RC955-962
Arctic
Canada
Online Access:https://doi.org/10.1590/0037-8682-0072-2018
https://doaj.org/article/5fec554b2f344aa992e9ff58b64dd678
Description
Summary:Abstract INTRODUCTION: Appropriate preservation of specimens is important for taxonomic identification. In sandfly research, various methods have been used for slide preparation; however, high cost, low commercial availability, and associated hazards make their use impossible in some studies. Therefore, the efficacy of Kisser glycerol gelatin for sandfly slide preparation was tested. METHODS: Kisser glycerol gelatin, as a substitute for Canada balsam and Berlese’s fluid, was used for mounting sandflies. RESULTS: Forty-two mounted specimens were created and maintained even after 14 months. CONCLUSIONS: Use of Kisser glycerol gelatin is simple and efficient for preparing microscope slides of sandflies.

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