Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus)

Abstract Objective The objectives of this work are to isolate, develop, and characterize polymorphic microsatellite markers for use in Greenland sharks (Somniosus microcephalus). Results Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 36 S. microcephalu...

Full description

Bibliographic Details
Published in:BMC Research Notes
Main Authors: Meaghan A. Swintek, Ryan P. Walter
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2021
Subjects:
Somniosus
Greenland shark
Population genetics
Microsatellites
Sleeper shark
Medicine
R
Biology (General)
QH301-705.5
Science (General)
Q1-390
Greenland
Pacific
Svalbard
Somniosus microcephalus
Online Access:https://doi.org/10.1186/s13104-021-05447-5
https://doaj.org/article/5aff85f8ba19427abc2f67f7c0190f0e
Description
Summary:Abstract Objective The objectives of this work are to isolate, develop, and characterize polymorphic microsatellite markers for use in Greenland sharks (Somniosus microcephalus). Results Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 36 S. microcephalus individuals from Grise Fjord (n = 16) and Svalbard (n = 20). Each locus yielded between 2 and 9 alleles and observed heterozygosity ranged from 0.11 to 0.70 when estimated across both sites. One locus and three loci deviated from HWE following Bonferroni correction, for individuals sampled from Grise Fjord and Svalbard, respectively. Cross-amplification was successful at every locus for five of the ten S. pacificus individuals.

Similar Items