A Pacific Oyster-Derived Antioxidant, DHMBA, Protects Renal Tubular HK-2 Cells against Oxidative Stress via Reduction of Mitochondrial ROS Production and Fragmentation
The kidney contains numerous mitochondria in proximal tubular cells that provide energy for tubular secretion and reabsorption. Mitochondrial injury and consequent excessive reactive oxygen species (ROS) production can cause tubular damage and play a major role in the pathogenesis of kidney diseases...
Published in: | International Journal of Molecular Sciences |
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ftdoajarticles:oai:doaj.org/article:5afdfb1211e640a7a242e79c52b5cf27 2023-07-23T04:18:56+02:00 A Pacific Oyster-Derived Antioxidant, DHMBA, Protects Renal Tubular HK-2 Cells against Oxidative Stress via Reduction of Mitochondrial ROS Production and Fragmentation Hsin-Jung Ho Natsumi Aoki Yi-Jou Wu Ming-Chen Gao Karin Sekine Toshihiro Sakurai Hitoshi Chiba Hideaki Watanabe Mitsugu Watanabe Shu-Ping Hui 2023-06-01T00:00:00Z https://doi.org/10.3390/ijms241210061 https://doaj.org/article/5afdfb1211e640a7a242e79c52b5cf27 EN eng MDPI AG https://www.mdpi.com/1422-0067/24/12/10061 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241210061 1422-0067 1661-6596 https://doaj.org/article/5afdfb1211e640a7a242e79c52b5cf27 International Journal of Molecular Sciences, Vol 24, Iss 10061, p 10061 (2023) mitochondria oxidative stress mitochondrial biogenesis mitochondrial fusion/fission mitophagy mitochondrial respiration Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms241210061 2023-07-02T00:38:08Z The kidney contains numerous mitochondria in proximal tubular cells that provide energy for tubular secretion and reabsorption. Mitochondrial injury and consequent excessive reactive oxygen species (ROS) production can cause tubular damage and play a major role in the pathogenesis of kidney diseases, including diabetic nephropathy. Accordingly, bioactive compounds that protect the renal tubular mitochondria from ROS are desirable. Here, we aimed to report 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), isolated from the Pacific oyster ( Crassostrea gigas ) as a potentially useful compound. In human renal tubular HK-2 cells, DHMBA significantly mitigated the cytotoxicity induced by the ROS inducer L-buthionine-(S, R)-sulfoximine (BSO). DHMBA reduced the mitochondrial ROS production and subsequently regulated mitochondrial homeostasis, including mitochondrial biogenesis, fusion/fission balance, and mitophagy; DHMBA also enhanced mitochondrial respiration in BSO-treated cells. These findings highlight the potential of DHMBA to protect renal tubular mitochondrial function against oxidative stress. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific International Journal of Molecular Sciences 24 12 10061 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
mitochondria oxidative stress mitochondrial biogenesis mitochondrial fusion/fission mitophagy mitochondrial respiration Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
mitochondria oxidative stress mitochondrial biogenesis mitochondrial fusion/fission mitophagy mitochondrial respiration Biology (General) QH301-705.5 Chemistry QD1-999 Hsin-Jung Ho Natsumi Aoki Yi-Jou Wu Ming-Chen Gao Karin Sekine Toshihiro Sakurai Hitoshi Chiba Hideaki Watanabe Mitsugu Watanabe Shu-Ping Hui A Pacific Oyster-Derived Antioxidant, DHMBA, Protects Renal Tubular HK-2 Cells against Oxidative Stress via Reduction of Mitochondrial ROS Production and Fragmentation |
topic_facet |
mitochondria oxidative stress mitochondrial biogenesis mitochondrial fusion/fission mitophagy mitochondrial respiration Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The kidney contains numerous mitochondria in proximal tubular cells that provide energy for tubular secretion and reabsorption. Mitochondrial injury and consequent excessive reactive oxygen species (ROS) production can cause tubular damage and play a major role in the pathogenesis of kidney diseases, including diabetic nephropathy. Accordingly, bioactive compounds that protect the renal tubular mitochondria from ROS are desirable. Here, we aimed to report 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), isolated from the Pacific oyster ( Crassostrea gigas ) as a potentially useful compound. In human renal tubular HK-2 cells, DHMBA significantly mitigated the cytotoxicity induced by the ROS inducer L-buthionine-(S, R)-sulfoximine (BSO). DHMBA reduced the mitochondrial ROS production and subsequently regulated mitochondrial homeostasis, including mitochondrial biogenesis, fusion/fission balance, and mitophagy; DHMBA also enhanced mitochondrial respiration in BSO-treated cells. These findings highlight the potential of DHMBA to protect renal tubular mitochondrial function against oxidative stress. |
format |
Article in Journal/Newspaper |
author |
Hsin-Jung Ho Natsumi Aoki Yi-Jou Wu Ming-Chen Gao Karin Sekine Toshihiro Sakurai Hitoshi Chiba Hideaki Watanabe Mitsugu Watanabe Shu-Ping Hui |
author_facet |
Hsin-Jung Ho Natsumi Aoki Yi-Jou Wu Ming-Chen Gao Karin Sekine Toshihiro Sakurai Hitoshi Chiba Hideaki Watanabe Mitsugu Watanabe Shu-Ping Hui |
author_sort |
Hsin-Jung Ho |
title |
A Pacific Oyster-Derived Antioxidant, DHMBA, Protects Renal Tubular HK-2 Cells against Oxidative Stress via Reduction of Mitochondrial ROS Production and Fragmentation |
title_short |
A Pacific Oyster-Derived Antioxidant, DHMBA, Protects Renal Tubular HK-2 Cells against Oxidative Stress via Reduction of Mitochondrial ROS Production and Fragmentation |
title_full |
A Pacific Oyster-Derived Antioxidant, DHMBA, Protects Renal Tubular HK-2 Cells against Oxidative Stress via Reduction of Mitochondrial ROS Production and Fragmentation |
title_fullStr |
A Pacific Oyster-Derived Antioxidant, DHMBA, Protects Renal Tubular HK-2 Cells against Oxidative Stress via Reduction of Mitochondrial ROS Production and Fragmentation |
title_full_unstemmed |
A Pacific Oyster-Derived Antioxidant, DHMBA, Protects Renal Tubular HK-2 Cells against Oxidative Stress via Reduction of Mitochondrial ROS Production and Fragmentation |
title_sort |
pacific oyster-derived antioxidant, dhmba, protects renal tubular hk-2 cells against oxidative stress via reduction of mitochondrial ros production and fragmentation |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms241210061 https://doaj.org/article/5afdfb1211e640a7a242e79c52b5cf27 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
International Journal of Molecular Sciences, Vol 24, Iss 10061, p 10061 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/24/12/10061 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241210061 1422-0067 1661-6596 https://doaj.org/article/5afdfb1211e640a7a242e79c52b5cf27 |
op_doi |
https://doi.org/10.3390/ijms241210061 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
12 |
container_start_page |
10061 |
_version_ |
1772181644442075136 |