Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon ( Salmon salar ) Exposed to Docosahexaenoic Acid
A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-l...
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MDPI AG
2023
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ftdoajarticles:oai:doaj.org/article:55b936793fc04e9fa2ac4956fb1258ed 2023-07-23T04:18:20+02:00 Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon ( Salmon salar ) Exposed to Docosahexaenoic Acid Pedro Araujo Sarah Iqbal Aleksander Arnø Marit Espe Elisabeth Holen 2023-06-01T00:00:00Z https://doi.org/10.3390/molecules28124728 https://doaj.org/article/55b936793fc04e9fa2ac4956fb1258ed EN eng MDPI AG https://www.mdpi.com/1420-3049/28/12/4728 https://doaj.org/toc/1420-3049 doi:10.3390/molecules28124728 1420-3049 https://doaj.org/article/55b936793fc04e9fa2ac4956fb1258ed Molecules, Vol 28, Iss 4728, p 4728 (2023) resolvins liquid chromatography quadrupole mass spectrometry Atlantic salmon head kidney cells liquid–liquid extraction Organic chemistry QD241-441 article 2023 ftdoajarticles https://doi.org/10.3390/molecules28124728 2023-07-02T00:37:31Z A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-level factorial design was proposed to select the optimal concentrations of internal standards that were used in the evaluation of the performance parameters, such as linear range (0.1–50 ng mL −1 ), limits of detection and quantification (0.05 and 0.1 ng mL −1 , respectively), and recovery values ranging from 96.9 to 99.8%. The optimized method was used to determine the stimulated production of resolvins by head kidney cells exposed to docosahexaenoic acid, and the results indicated that it is possible that the production was controlled by circadian responses. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Molecules 28 12 4728 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
resolvins liquid chromatography quadrupole mass spectrometry Atlantic salmon head kidney cells liquid–liquid extraction Organic chemistry QD241-441 |
spellingShingle |
resolvins liquid chromatography quadrupole mass spectrometry Atlantic salmon head kidney cells liquid–liquid extraction Organic chemistry QD241-441 Pedro Araujo Sarah Iqbal Aleksander Arnø Marit Espe Elisabeth Holen Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon ( Salmon salar ) Exposed to Docosahexaenoic Acid |
topic_facet |
resolvins liquid chromatography quadrupole mass spectrometry Atlantic salmon head kidney cells liquid–liquid extraction Organic chemistry QD241-441 |
description |
A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-level factorial design was proposed to select the optimal concentrations of internal standards that were used in the evaluation of the performance parameters, such as linear range (0.1–50 ng mL −1 ), limits of detection and quantification (0.05 and 0.1 ng mL −1 , respectively), and recovery values ranging from 96.9 to 99.8%. The optimized method was used to determine the stimulated production of resolvins by head kidney cells exposed to docosahexaenoic acid, and the results indicated that it is possible that the production was controlled by circadian responses. |
format |
Article in Journal/Newspaper |
author |
Pedro Araujo Sarah Iqbal Aleksander Arnø Marit Espe Elisabeth Holen |
author_facet |
Pedro Araujo Sarah Iqbal Aleksander Arnø Marit Espe Elisabeth Holen |
author_sort |
Pedro Araujo |
title |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon ( Salmon salar ) Exposed to Docosahexaenoic Acid |
title_short |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon ( Salmon salar ) Exposed to Docosahexaenoic Acid |
title_full |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon ( Salmon salar ) Exposed to Docosahexaenoic Acid |
title_fullStr |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon ( Salmon salar ) Exposed to Docosahexaenoic Acid |
title_full_unstemmed |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon ( Salmon salar ) Exposed to Docosahexaenoic Acid |
title_sort |
validation of a liquid–liquid extraction method to study the temporal production of d-series resolvins by head kidney cells from atlantic salmon ( salmon salar ) exposed to docosahexaenoic acid |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/molecules28124728 https://doaj.org/article/55b936793fc04e9fa2ac4956fb1258ed |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Molecules, Vol 28, Iss 4728, p 4728 (2023) |
op_relation |
https://www.mdpi.com/1420-3049/28/12/4728 https://doaj.org/toc/1420-3049 doi:10.3390/molecules28124728 1420-3049 https://doaj.org/article/55b936793fc04e9fa2ac4956fb1258ed |
op_doi |
https://doi.org/10.3390/molecules28124728 |
container_title |
Molecules |
container_volume |
28 |
container_issue |
12 |
container_start_page |
4728 |
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1772180596939816960 |