Molecular typing of Candida albicans strains isolated from nosocomial candidemia Tipificação molecular de espécies de Candida albicans isoladas de candidemia hospitalar

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Maria Luiza Moretti Branchini, Débora de Cassia Pires Geiger, Olga Fischman, Antonio Carlos Pignatari
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1995
• Subjects: Candida albicans; Nosocomial candidemia; Pulsed-field gel electrophoresis; Molecular typing; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46651995000600002; https://doaj.org/article/54096caa3f984b7f9957c77dc148fea5

Yeasts of the genus Candida have been recognized as important microorganisms responsible for nosocomial fungemia. Six blood-stream and two intravenous central catheter C. albicans strains were isolated from eight patients and studied by electrophoretic karyotyping of chromosomal DNA by pulsed-field gel electrophoresis. Seven chromosomal DNA profiles were identified. Two patients showed isolates with the same profile, suggesting nosocomial transmission. Karyotyping of C. albicans revealed an excellent discriminatory power among the isolates and may therefore be useful in the study of nosocomial candidemia. Leveduras do gênero Candida têm sido reconhecidas como importantes causadoras de fungemias hospitalares. Foram estudados os DNA cromossômicos de oito cepas de C. albicans, obtidas de oito pacientes com fungemia hospitalar, por cariotipagem eletroforética através de "pulsed-field gel electrophoresis". As cepas foram obtidas pelo isolamento da levedura em seis hemoculturas e duas infecções relacionadas ao uso de cateter intra-venoso central. Foram identificados sete perfis de DNA cromossômico. Dois pacientes mostraram cepas com o mesmo perfil de DNA sugerindo transmissão nosocomial. A cariotipagem eletroforética revelou excelente capacidade discriminatória entre os isolados sendo útil no estudo das candidemias hospitalares.