Molecular typing of Candida albicans strains isolated from nosocomial candidemia Tipificação molecular de espécies de Candida albicans isoladas de candidemia hospitalar

Yeasts of the genus Candida have been recognized as important microorganisms responsible for nosocomial fungemia. Six blood-stream and two intravenous central catheter C. albicans strains were isolated from eight patients and studied by electrophoretic karyotyping of chromosomal DNA by pulsed-field...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Maria Luiza Moretti Branchini, Débora de Cassia Pires Geiger, Olga Fischman, Antonio Carlos Pignatari
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 1995
Subjects:
Candida albicans
Nosocomial candidemia
Pulsed-field gel electrophoresis
Molecular typing
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/S0036-46651995000600002
https://doaj.org/article/54096caa3f984b7f9957c77dc148fea5
Description
Summary:Yeasts of the genus Candida have been recognized as important microorganisms responsible for nosocomial fungemia. Six blood-stream and two intravenous central catheter C. albicans strains were isolated from eight patients and studied by electrophoretic karyotyping of chromosomal DNA by pulsed-field gel electrophoresis. Seven chromosomal DNA profiles were identified. Two patients showed isolates with the same profile, suggesting nosocomial transmission. Karyotyping of C. albicans revealed an excellent discriminatory power among the isolates and may therefore be useful in the study of nosocomial candidemia. Leveduras do gênero Candida têm sido reconhecidas como importantes causadoras de fungemias hospitalares. Foram estudados os DNA cromossômicos de oito cepas de C. albicans, obtidas de oito pacientes com fungemia hospitalar, por cariotipagem eletroforética através de "pulsed-field gel electrophoresis". As cepas foram obtidas pelo isolamento da levedura em seis hemoculturas e duas infecções relacionadas ao uso de cateter intra-venoso central. Foram identificados sete perfis de DNA cromossômico. Dois pacientes mostraram cepas com o mesmo perfil de DNA sugerindo transmissão nosocomial. A cariotipagem eletroforética revelou excelente capacidade discriminatória entre os isolados sendo útil no estudo das candidemias hospitalares.

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