In vitro vasodilatory effect of aqueous leaf extract of Thymus serrulatus on thoracic aorta of Guinea pigs

Objective: To investigate the vasodilatory effect of Thymus serrulatus (T. serrulatus) aqueous leaf extract on KCl (high K+, 80 mmol/L) induced precontracted isolated thoracic aorta rings on guinea pigs and the role of aorta endothelium on this action. Methods: Guinea pig thoracic aorta was removed...

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Bibliographic Details
Published in:Asian Pacific Journal of Tropical Biomedicine
Main Authors: Bekesho Geleta, Mebrahtu Eyasu, Selamu Kebamo, Asfaw Debella, Eyasu Makonnen, Abiy Abebe
Format: Article in Journal/Newspaper
Language:English
Published: Wolters Kluwer Medknow Publications 2015
Subjects:
Thymus serrulatus
Guinea pig
Aqueous extract
Endothelium
Organ bath
Vasodilatory effect
Arctic medicine. Tropical medicine
RC955-962
Biology (General)
QH301-705.5
Arctic
Krebs
Online Access:https://doi.org/10.1016/S2221-1691(15)30164-7
https://doaj.org/article/53e5dafd07214787bdcbcb745aed4193
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Summary:Objective: To investigate the vasodilatory effect of Thymus serrulatus (T. serrulatus) aqueous leaf extract on KCl (high K+, 80 mmol/L) induced precontracted isolated thoracic aorta rings on guinea pigs and the role of aorta endothelium on this action. Methods: Guinea pig thoracic aorta was removed and placed in an organ bath containing Krebs-Henseleit solution and aorta contractions were recorded isometrically. Results: The results revealed that T. serrulatus aqueous leaf extract (0.5-5 mg/mL) significantly (P<0.001) reduced KCl-induced contractions of guinea pig thoracic aorta in both intact (n=5) and denuded (n=5) endothelium in a concentration dependent manner, and the vasodilatory effect of the extract on intact endothelium was significantly (P<0.05) higher than that on denuded endothelium. Glibenclamide (10 µmol/L) significantly (P<0.001) increased the vasodilatory effect of extract in intact endothelium as compared to methylene blue (10 µmol/L), atropine (10 µmol/L) and indomethacin (10 µmol/L). The effect was more obvious on intact than that on denuded endothelium. Conclusions: The present findings demonstrate that T. serrulatus aqueous leaf extract has vasodilatory activity which might result in antihypertensive effect and its vasodilatory effect is endothelium-dependent. This might support the traditional claim of the plant in hypertensive.

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