Laminaria hyperborea as a Source of Valuable Glyceroglycolipids—A Characterization of Galactosyldiacilglycerols in Stipe and Blade by HPLC-MS/MS
Laminaria hyperborea (Gunnerus) Foslie 1885 is a seaweed native to the North Atlantic, which is utilized in the production of alginate. Its potential as a source of bioactive lipids remains unexplored. In this study, mono- and digalactosyldiacylglycerols (MGDG and DGDG) were identified in stipe and...
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MDPI AG
2022
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ftdoajarticles:oai:doaj.org/article:53bbf6f896a84024a425b25b06c1182a 2023-05-15T17:34:57+02:00 Laminaria hyperborea as a Source of Valuable Glyceroglycolipids—A Characterization of Galactosyldiacilglycerols in Stipe and Blade by HPLC-MS/MS Lena Foseid Hanne Devle Carl Fredrik Naess-Andresen Dag Ekeberg 2022-09-01T00:00:00Z https://doi.org/10.3390/appliedchem2040013 https://doaj.org/article/53bbf6f896a84024a425b25b06c1182a EN eng MDPI AG https://www.mdpi.com/2673-9623/2/4/13 https://doaj.org/toc/2673-9623 doi:10.3390/appliedchem2040013 2673-9623 https://doaj.org/article/53bbf6f896a84024a425b25b06c1182a AppliedChem, Vol 2, Iss 13, Pp 185-198 (2022) glyceroglycolipids monogalactosyldiacylglycerol digalactosyldiacylglycerol HPLC-MS/MS GC-MS seaweed Analytical chemistry QD71-142 General. Including alchemy QD1-65 article 2022 ftdoajarticles https://doi.org/10.3390/appliedchem2040013 2023-02-26T01:31:31Z Laminaria hyperborea (Gunnerus) Foslie 1885 is a seaweed native to the North Atlantic, which is utilized in the production of alginate. Its potential as a source of bioactive lipids remains unexplored. In this study, mono- and digalactosyldiacylglycerols (MGDG and DGDG) were identified in stipe and blade from L. hyperborea for the first time. Samples were harvested off the west coast of Norway in May 2018. Lipids were extracted with chloroform:methanol (2:1, v / v ) and fractionated using solid phase extraction, whereupon the fatty acid content was determined by gas chromatography-mass spectrometry. The fatty acid profile was used to predict the mass of the glyceroglycolipids. A total of 103 and 161 molecular species of MGDG, and 66 and 136 molecular species of DGDG were identified in blade and stipe, respectively, by HPLC-ESI-MS/MS. The most abundant molecular species were identified from the total ion chromatograms. According to these, MGDG(20:5/18:4, 18:4/18:4, 16:0/18:1, 14:0/18:2, 14:0/18:1) and DGDG(20:5/18:4, 16:0/18:1, 14:0/18:1) were the most abundant in blade. On the other hand, in stipe, the most abundant molecular species were MGDG (14:0/18:2, 14:0/18:1, 16:0/18:1) and DGDG (14:0/18:1). The purpose of this study is to highlight the potential application of L. hyperborea in a biotechnological context. Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Norway AppliedChem 2 4 185 198 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
glyceroglycolipids monogalactosyldiacylglycerol digalactosyldiacylglycerol HPLC-MS/MS GC-MS seaweed Analytical chemistry QD71-142 General. Including alchemy QD1-65 |
spellingShingle |
glyceroglycolipids monogalactosyldiacylglycerol digalactosyldiacylglycerol HPLC-MS/MS GC-MS seaweed Analytical chemistry QD71-142 General. Including alchemy QD1-65 Lena Foseid Hanne Devle Carl Fredrik Naess-Andresen Dag Ekeberg Laminaria hyperborea as a Source of Valuable Glyceroglycolipids—A Characterization of Galactosyldiacilglycerols in Stipe and Blade by HPLC-MS/MS |
topic_facet |
glyceroglycolipids monogalactosyldiacylglycerol digalactosyldiacylglycerol HPLC-MS/MS GC-MS seaweed Analytical chemistry QD71-142 General. Including alchemy QD1-65 |
description |
Laminaria hyperborea (Gunnerus) Foslie 1885 is a seaweed native to the North Atlantic, which is utilized in the production of alginate. Its potential as a source of bioactive lipids remains unexplored. In this study, mono- and digalactosyldiacylglycerols (MGDG and DGDG) were identified in stipe and blade from L. hyperborea for the first time. Samples were harvested off the west coast of Norway in May 2018. Lipids were extracted with chloroform:methanol (2:1, v / v ) and fractionated using solid phase extraction, whereupon the fatty acid content was determined by gas chromatography-mass spectrometry. The fatty acid profile was used to predict the mass of the glyceroglycolipids. A total of 103 and 161 molecular species of MGDG, and 66 and 136 molecular species of DGDG were identified in blade and stipe, respectively, by HPLC-ESI-MS/MS. The most abundant molecular species were identified from the total ion chromatograms. According to these, MGDG(20:5/18:4, 18:4/18:4, 16:0/18:1, 14:0/18:2, 14:0/18:1) and DGDG(20:5/18:4, 16:0/18:1, 14:0/18:1) were the most abundant in blade. On the other hand, in stipe, the most abundant molecular species were MGDG (14:0/18:2, 14:0/18:1, 16:0/18:1) and DGDG (14:0/18:1). The purpose of this study is to highlight the potential application of L. hyperborea in a biotechnological context. |
format |
Article in Journal/Newspaper |
author |
Lena Foseid Hanne Devle Carl Fredrik Naess-Andresen Dag Ekeberg |
author_facet |
Lena Foseid Hanne Devle Carl Fredrik Naess-Andresen Dag Ekeberg |
author_sort |
Lena Foseid |
title |
Laminaria hyperborea as a Source of Valuable Glyceroglycolipids—A Characterization of Galactosyldiacilglycerols in Stipe and Blade by HPLC-MS/MS |
title_short |
Laminaria hyperborea as a Source of Valuable Glyceroglycolipids—A Characterization of Galactosyldiacilglycerols in Stipe and Blade by HPLC-MS/MS |
title_full |
Laminaria hyperborea as a Source of Valuable Glyceroglycolipids—A Characterization of Galactosyldiacilglycerols in Stipe and Blade by HPLC-MS/MS |
title_fullStr |
Laminaria hyperborea as a Source of Valuable Glyceroglycolipids—A Characterization of Galactosyldiacilglycerols in Stipe and Blade by HPLC-MS/MS |
title_full_unstemmed |
Laminaria hyperborea as a Source of Valuable Glyceroglycolipids—A Characterization of Galactosyldiacilglycerols in Stipe and Blade by HPLC-MS/MS |
title_sort |
laminaria hyperborea as a source of valuable glyceroglycolipids—a characterization of galactosyldiacilglycerols in stipe and blade by hplc-ms/ms |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/appliedchem2040013 https://doaj.org/article/53bbf6f896a84024a425b25b06c1182a |
geographic |
Norway |
geographic_facet |
Norway |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
AppliedChem, Vol 2, Iss 13, Pp 185-198 (2022) |
op_relation |
https://www.mdpi.com/2673-9623/2/4/13 https://doaj.org/toc/2673-9623 doi:10.3390/appliedchem2040013 2673-9623 https://doaj.org/article/53bbf6f896a84024a425b25b06c1182a |
op_doi |
https://doi.org/10.3390/appliedchem2040013 |
container_title |
AppliedChem |
container_volume |
2 |
container_issue |
4 |
container_start_page |
185 |
op_container_end_page |
198 |
_version_ |
1766133957045256192 |