Seed Germination after 30 Years Storage in Permafrost

More than 30 years ago, the Nordic Gene Bank established a long-term experiment on seeds stored under permafrost conditions in an abandoned mine corridor in Svalbard, as a tool to monitor storage life under these conditions. The study included seeds from 16 Nordic agricultural and horticultural crop...

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Bibliographic Details
Published in:Plants
Main Authors: Svein Øivind Solberg, Guro Brodal, Roland von Bothmer, Eivind Meen, Flemming Yndgaard, Christian Andreasen, Åsmund Asdal
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2020
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Online Access:https://doi.org/10.3390/plants9050579
https://doaj.org/article/5175518b8fe94594a786f3e6f956f2a7
Description
Summary:More than 30 years ago, the Nordic Gene Bank established a long-term experiment on seeds stored under permafrost conditions in an abandoned mine corridor in Svalbard, as a tool to monitor storage life under these conditions. The study included seeds from 16 Nordic agricultural and horticultural crops, each represented by two or three cultivars (altogether 38 accessions). All seeds were ultra-dried to 3%–5% moisture before being sealed in glass tubes. Germination tests were performed in accordance with the International Seed Testing Association (ISTA) protocols. At the initiation of the experiment, the samples showed good germination with the median value at 92%. The overall picture remained stable over the first twenty to twenty-five years. However, the variation became larger over time and at 30 years, the median value had dropped to 80%. At the lower end, with a high drop in germination, we found rye, wheat, and English ryegrass. At the upper end, we found Kentucky bluegrass and cucumber. The lowest germination was found in samples with the highest initial seed moisture levels. Pre-storage conditions are likely to be of major importance for longevity.