Screening of Antimicrobial Resistance Genes and Epidemiological Features in Hospital and Community-Associated Carbapenem-Resistant Pseudomonas aeruginosa Infections

• Main Authors: Çopur Çiçek A, Ertürk A, Ejder N, Rakici E, Kostakoğlu U, Esen Yıldız İ, Özyurt S, Sönmez E
• Format: Article in Journal/Newspaper
• Language: English
• Published: Dove Medical Press 2021
• Subjects: pseudomonas aeruginosa; antibiotic resistance genes; epidemiology; pfge; Infectious and parasitic diseases; RC109-216; ejder
• Online Access: https://doaj.org/article/50d6b174460e409eb4d1cca98b4d14ff

Ayşegül Çopur Çiçek,1 Ayşe Ertürk,2 Nebahat Ejder,1 Erva Rakici,1 Uğur Kostakoğlu,2 İlknur Esen Yıldız,2 Songül Özyurt,3 Emine Sönmez2 1Recep Tayyip Erdogan University, Faculty of Medicine, Department of Medical Microbiology, Rize, Turkey; 2Recep Tayyip Erdogan University, Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Rize, Turkey; 3Recep Tayyip Erdogan University, Faculty of Medicine, Department of Chest Diseases, Rize, TurkeyCorrespondence: Ayşegül Çopur ÇiçekRecep Tayyip Erdogan University, Faculty of Medicine, Department of Medical Microbiology, Rize, 53100, TurkeyEmail [e-mail]: Researching carbapenem-resistant isolates enables the identification of carbapenemase-producing bacteria and prevents their spread.Methods: P. aeruginosa isolates were recovered from Medicine Faculty of Recep Tayyip Erdoğan University and identified by conventional methods and the automated Vitek 2 Compact system. Antimicrobial susceptibility experiments were performed in accordance with CLSI criteria and the automated Vitek 2 Compact system. The PCR method was investigated for the presence of β-lactamase resistance genes. PFGE typing was performed to show clonal relation among samples.Results: Seventy P. aeruginosa isolates were isolated from seventy patients. Of the patients, 67.1% had contact with the health service in the last 90 days and 75.7% of the patients had received antimicrobial therapy in the previous 90 days. Twenty-four isolates were carbapenem resistant, 2 isolates were multidrug-resistant except colistin, and none of the samples had colistin resistance. The gene encoding β-lactamase or metallo-β-lactamase was found in a total of 36 isolates. The blaVEB and blaPER genes were identified in 1 and 5 isolates alone or 17 and 13 isolates in combination with other resistance genes, respectively. The blaNDM was the most detected metallo-β-lactamase encoding gene (n=18), followed by blaKPC (n=12). blaIMP and blaVIM were detected in 5 and 1 isolates, respectively. ...