Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice

Abstract INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS Food samples were experimentally contaminated with insects, pro...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical
Main Authors: Elaine Cristina de Mattos, Maria Aparecida Moraes Marciano, Vilma dos Santos Menezes Gaiotto Daros, Cristiane Castro Faccini, Angela Maria Lourenço, Vera Lucia Pereira-Chioccola
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2019
Subjects:
Coleoptera
Euterpe
Triatominae
Polymerase Chain Reaction
Food
Foodborne Diseases
Arctic medicine. Tropical medicine
RC955-962
Arctic
Online Access:https://doi.org/10.1590/0037-8682-0119-2019
https://doaj.org/article/4f277c21d11c419a8cdf5e43ae2a47c2
Description
Summary:Abstract INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR). RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food. CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples.

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