A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae

The gal80 mutant of yeast Saccharomyces cerevisiae is used for the constitutive expression under strong GAL promoters without galactose induction. To enhance productivity of gal80 mutant, an alternative strain, allgal, was developed by removing all galactose-utilizing genes that consume significant...

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Bibliographic Details
Published in:Biotechnology Reports
Main Authors: Mi-Jin Kim, Bong Hyun Sung, Hyun-Joo Park, Jung-Hoon Sohn, Jung-Hoon Bae
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2022
Subjects:
Galactose
GAL10 promoter
Recombinant proteins
Constitutive expression
Saccharomyces cerevisiae
Biotechnology
TP248.13-248.65
Antarc*
Antarctica
Online Access:https://doi.org/10.1016/j.btre.2022.e00763
https://doaj.org/article/4e7a03336f3a449f90ddc4763b53d6c6
Description
Summary:The gal80 mutant of yeast Saccharomyces cerevisiae is used for the constitutive expression under strong GAL promoters without galactose induction. To enhance productivity of gal80 mutant, an alternative strain, allgal, was developed by removing all galactose-utilizing genes that consume significant cellular resources in the gal80 strain when cultured in non-galactose conditions. The efficacy of the allgal mutant (gal80, gal1, gal2, gal7, and gal10) was verified by assessing the secretory expression of three recombinant proteins, Candida antarctica lipase B (CalB), human serum albumin (HSA), and human epidermal growth factor (hEGF), using the GAL10 promoter. The growth of the allgal mutant was enhanced by 15–38% compared to the gal80 mutant, and the secretion of recombinant proteins also increased by 16–22% in fed-batch fermentation. Thus, the expression of recombinant proteins using GAL10 promoter in the allgal mutant is suitable for the economical production of recombinant proteins in S. cerevisiae.

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