A simple PCR-based procedure for plaque diagnosis Um método simples para o diagnóstico de peste por PCR

Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals. Tr...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Nilma Cintra Leal, Frederico Guilherme Coutinho Abath, Luis Carlos Alves, Alzira Maria Paiva de Almeida
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 1996
Subjects:
Plague
Yersinia pestis
Rodents
PCR
Diagnosis
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/S0036-46651996000500009
https://doaj.org/article/4d35f5957ddf432aac95187ce50d8eb4
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spelling ftdoajarticles:oai:doaj.org/article:4d35f5957ddf432aac95187ce50d8eb4 2024-09-09T19:24:19+00:00 A simple PCR-based procedure for plaque diagnosis Um método simples para o diagnóstico de peste por PCR Nilma Cintra Leal Frederico Guilherme Coutinho Abath Luis Carlos Alves Alzira Maria Paiva de Almeida 1996-10-01T00:00:00Z https://doi.org/10.1590/S0036-46651996000500009 https://doaj.org/article/4d35f5957ddf432aac95187ce50d8eb4 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651996000500009 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651996000500009 0036-4665 1678-9946 https://doaj.org/article/4d35f5957ddf432aac95187ce50d8eb4 Revista do Instituto de Medicina Tropical de São Paulo, Vol 38, Iss 5, Pp 371-374 (1996) Plague Yersinia pestis Rodents PCR Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1996 ftdoajarticles https://doi.org/10.1590/S0036-46651996000500009 2024-08-05T17:49:31Z Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals. Triturados de baços de animais infectados experimentalmente com Y. pestis, suspensos em salina foram fervidos e os sobrenadantes usados diretamente para amplificação do PCR sem prévia extração do DNA. O limiar de detecção poda ser aumentado por uma segunda etapa de amplificação (Nested-PCR). Não houve amplificação a partir das amostras dos animais não infectados usados como controle. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 38 5 371 374
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Plague
Yersinia pestis
Rodents
PCR
Diagnosis
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle Plague
Yersinia pestis
Rodents
PCR
Diagnosis
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Nilma Cintra Leal
Frederico Guilherme Coutinho Abath
Luis Carlos Alves
Alzira Maria Paiva de Almeida
A simple PCR-based procedure for plaque diagnosis Um método simples para o diagnóstico de peste por PCR
topic_facet Plague
Yersinia pestis
Rodents
PCR
Diagnosis
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals. Triturados de baços de animais infectados experimentalmente com Y. pestis, suspensos em salina foram fervidos e os sobrenadantes usados diretamente para amplificação do PCR sem prévia extração do DNA. O limiar de detecção poda ser aumentado por uma segunda etapa de amplificação (Nested-PCR). Não houve amplificação a partir das amostras dos animais não infectados usados como controle.
format Article in Journal/Newspaper
author Nilma Cintra Leal
Frederico Guilherme Coutinho Abath
Luis Carlos Alves
Alzira Maria Paiva de Almeida
author_facet Nilma Cintra Leal
Frederico Guilherme Coutinho Abath
Luis Carlos Alves
Alzira Maria Paiva de Almeida
author_sort Nilma Cintra Leal
title A simple PCR-based procedure for plaque diagnosis Um método simples para o diagnóstico de peste por PCR
title_short A simple PCR-based procedure for plaque diagnosis Um método simples para o diagnóstico de peste por PCR
title_full A simple PCR-based procedure for plaque diagnosis Um método simples para o diagnóstico de peste por PCR
title_fullStr A simple PCR-based procedure for plaque diagnosis Um método simples para o diagnóstico de peste por PCR
title_full_unstemmed A simple PCR-based procedure for plaque diagnosis Um método simples para o diagnóstico de peste por PCR
title_sort simple pcr-based procedure for plaque diagnosis um método simples para o diagnóstico de peste por pcr
publisher Universidade de São Paulo (USP)
publishDate 1996
url https://doi.org/10.1590/S0036-46651996000500009
https://doaj.org/article/4d35f5957ddf432aac95187ce50d8eb4
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Revista do Instituto de Medicina Tropical de São Paulo, Vol 38, Iss 5, Pp 371-374 (1996)
op_relation http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651996000500009
https://doaj.org/toc/0036-4665
https://doaj.org/toc/1678-9946
doi:10.1590/S0036-46651996000500009
0036-4665
1678-9946
https://doaj.org/article/4d35f5957ddf432aac95187ce50d8eb4
op_doi https://doi.org/10.1590/S0036-46651996000500009
container_title Revista do Instituto de Medicina Tropical de São Paulo
container_volume 38
container_issue 5
container_start_page 371
op_container_end_page 374
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