A simple PCR-based procedure for plaque diagnosis Um método simples para o diagnóstico de peste por PCR

Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals. Tr...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Nilma Cintra Leal, Frederico Guilherme Coutinho Abath, Luis Carlos Alves, Alzira Maria Paiva de Almeida
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 1996
Subjects:
Plague
Yersinia pestis
Rodents
PCR
Diagnosis
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/S0036-46651996000500009
https://doaj.org/article/4d35f5957ddf432aac95187ce50d8eb4
Description
Summary:Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals. Triturados de baços de animais infectados experimentalmente com Y. pestis, suspensos em salina foram fervidos e os sobrenadantes usados diretamente para amplificação do PCR sem prévia extração do DNA. O limiar de detecção poda ser aumentado por uma segunda etapa de amplificação (Nested-PCR). Não houve amplificação a partir das amostras dos animais não infectados usados como controle.

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