How prevalent is Plasmodium malariae in Rondônia, Western Brazilian Amazon?

We have compared results of Plasmodium species identification obtained with conventional on-site microscopy of Giemsa-stained thick smears (GTS) and a semi-nested polymerase chain reaction (PCR) in 96 malaria patients from Rondônia, Western Brazilian Amazon. Mixed-species infections were detected by...

Full description

Bibliographic Details
Main Authors: Marisa Torres Vidal Cavasini, Weber Luidi Ribeiro, Fumihiko Kawamoto, Marcelo Urbano Ferreira
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2000
Subjects:
PCR
Online Access:https://doaj.org/article/4777264d70c34a8c96ac34d6b6f3a29a
Description
Summary:We have compared results of Plasmodium species identification obtained with conventional on-site microscopy of Giemsa-stained thick smears (GTS) and a semi-nested polymerase chain reaction (PCR) in 96 malaria patients from Rondônia, Western Brazilian Amazon. Mixed-species infections were detected by PCR in 30% patients, but no such case had been found on GTS. Moreover, P. malariae infections were detected in 9 of 96 patients (10%) by PCR, but were not identified by local microscopists. The potential impact of species misidentification on malaria treatment and control is discussed.