Methicillin-resistant Staphylococcus aureus DNA electrophoretic pattern: temporal changes in an endemic hospital environment Patrón electroforético del ADN de Staphylococcus aureus resistente a la meticilina: cambios temporales en un medio hospitalario endémico

• Main Authors: Gleice Cristina Leite, Maria Clara Padoveze, Maria Luiza Moretti
• Format: Article in Journal/Newspaper
• Language: English; Spanish; Portuguese
• Published: Pan American Health Organization 2011
• Subjects: Staphylococcus aureus; Staphylococcus aureus resistente a meticilina; electroforesis en gel de campo pulsado; farmacorresistencia microbiana; Brasil; methicillin-resistant Staphylococcus aureus; electrophoresis; gel; pulsed-field; drug resistance; microbial; Brazil; Medicine; R; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Arctic
• Online Access: https://doaj.org/article/42946b5b3bdb4465a8edf992771e1088

OBJECTIVE: To describe the analysis of geographical and temporal distribution of DNA profiles determined by pulsedfield gel electrophoresis (PFGE) of methicillinresistant Staphylococcus aureus (MRSA) strains isolated from hospitalized patients in a tertiary care university hospital in Brazil. METHODS: Ninetynine samples of MRSA obtained from 89 patients in the period 1999- 2004 were studied. MRSA strains were isolated from central venous catheters (33 isolates) and bloodstream infections (66 strains). PFGE with 20 units of SmaI restriction endonuclease was used for genomic typing. RESULTS: Analysis of DNA PFGE of 99 strains of MRSA revealed 26 profiles and their respective related profiles. The mean time interval for detecting MRSA infection was 26 days from hospital admission. Forty-nine patients (57.6%) had a recent hospitalization. The DNA PFGE MRSA profiles were distributed in three clonal groups-I, II, and III-according to the period of time when the MRSA strains were isolated. DNA PFGE MRSA profiles were spread homogeneously through all hospital wards. CONCLUSIONS: Changes in the distribution of DNA PFGE MRSA profiles were largely temporal, with clonal groups being replaced over time, without predominance in any hospital ward or any specific area of the hospital. OBJETIVO: Analizar la distribución geográfica y temporal de los perfiles de ADN determinados mediante electroforesis en gel de campo pulsado (PFGE) de cepas de Staphylococcus aureus resistente a la meticilina (SARM) aisladas de pacientes internados en un hospital universitario de atención terciaria en el Brasil. MÉTODOS: Se estudiaron 99 muestras de SARM obtenidas 89 de pacientes en el período 1999-2004. Las cepas de SARM se aislaron de infecciones de catéteres venosos centrales (33 aislados) y del torrente sanguíneo (66 cepas). Para la tipificación genómica se empleó PFGE con 20 unidades de endonucleasa de restricción SmaI. RESULTADOS: El análisis del ADN de 99 cepas de SARM mediante PFGE reveló 26 perfiles, con sus respectivos perfiles ...