Synthesis of Mesoporous Silica Nanowires and Their Application in Enzyme Immobilization

Hydrophobic mesoporous silica nanowires were synthesis and then employed as support for immobilization of lipase from Candida antarctica via covalent bonding (CALB@MSW). The parameters were optimized and the optimum conditions were as follows: GA concentration 5.5 wt.%, activation time 60 min and CA...

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Bibliographic Details
Published in:E3S Web of Conferences
Main Authors: Liu Peng, Liu Xinlong, An Na, Wang Peng
Format: Article in Journal/Newspaper
Language:English
French
Published: EDP Sciences 2021
Subjects:
Environmental sciences
GE1-350
Antarc*
Antarctica
Online Access:https://doi.org/10.1051/e3sconf/202124503006
https://doaj.org/article/4141a34be5fb45dd9a13490f118df817
Description
Summary:Hydrophobic mesoporous silica nanowires were synthesis and then employed as support for immobilization of lipase from Candida antarctica via covalent bonding (CALB@MSW). The parameters were optimized and the optimum conditions were as follows: GA concentration 5.5 wt.%, activation time 60 min and CALB concentration 4 mg/mL. Under these conditions, the protein loading and specific activity of CALB@MSW were 138.3 mg/gsupport and 41.1 U/mgsupport, respectively. Compared with free CALB, CALB@MSW showed better thermal stability and pH stability. The maximum yield of biodiesel catalytic by CALB@MSW was 93.4 %. After reused 8 times, CALB@MSW still remained 95.75 % initial activity showing better stability than free CALB.

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