The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome

Abstract We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small s...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical
Main Authors: Daniella de Sousa Mendes Moreira Alves, Gustavo Souza Gonçalves, Aline Silva Moraes, Luciano Moreira Alves, José Rodrigues do Carmo Neto, Mariana Machado Hecht, Nadjar Nitz, Rodrigo Gurgel-Gonçalves, Geisa Bernardes, Ana Maria de Castro, Maria Regina Chalita, Marina Clare Vinaud
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT)
Subjects:
Acanthamoeba keratitis
Culture
PCR
Arctic medicine. Tropical medicine
RC955-962
Arctic
Online Access:https://doi.org/10.1590/0037-8682-0010-2018
https://doaj.org/article/3fc011f6ec8f4a0b988f042391ced23b
Description
Summary:Abstract We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit rDNA; both culture and PCR were positive. The sequence analyses of the cornea and of isolates from the contact lens case showed similarity with the T4 genotype. To the best of our knowledge, this is the first report of T4 Acanthamoeba keratitis case from the Midwest region of Brazil.

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