An immunohistochemic assay to localize leptospires in tissue specimens

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Venâncio A. F. Alves, Paulo H. Yasuda, Edite H. Yamashiro, Raimunda Telma M. Santos, Luzia Umeda Yamamoto, Thales de Brito
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1986
• Subjects: Leptospirosis; Immunodiagnosis; Immunohistochemistry; Kidney Sections; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46651986000300006; https://doaj.org/article/3ef09ff190ff44ad990e30793b087d40

An Immunoperoxidase technique for identification of leptospires in formalin fixed, paraffin embedded kidney sections is presented, using peroxidase-antiperoxidase complex. The anti-leptospiral antibody was raised in rabbit. Possible applications of this technique are discussed.