An immunohistochemic assay to localize leptospires in tissue specimens

An Immunoperoxidase technique for identification of leptospires in formalin fixed, paraffin embedded kidney sections is presented, using peroxidase-antiperoxidase complex. The anti-leptospiral antibody was raised in rabbit. Possible applications of this technique are discussed.

Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Venâncio A. F. Alves, Paulo H. Yasuda, Edite H. Yamashiro, Raimunda Telma M. Santos, Luzia Umeda Yamamoto, Thales de Brito
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 1986
Subjects:
Leptospirosis
Immunodiagnosis
Immunohistochemistry
Kidney Sections
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/S0036-46651986000300006
https://doaj.org/article/3ef09ff190ff44ad990e30793b087d40
Description
Summary:An Immunoperoxidase technique for identification of leptospires in formalin fixed, paraffin embedded kidney sections is presented, using peroxidase-antiperoxidase complex. The anti-leptospiral antibody was raised in rabbit. Possible applications of this technique are discussed.

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