Neutrophil extracellular traps in the intestinal mucosa of Eimeria-infected animals
Objective: To investigate the presence of neutrophil extracellular traps (NETs) in vivo by analysing intestinal sections from experimentally Eimeria bovis- and naturally Eimeria arloingi-infected animals. Methods: Intestinal samples of Eimeria arloingi- and Eimeria bovis-infected animals were analys...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2016
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Subjects: | |
Online Access: | https://doi.org/10.1016/j.apjtb.2016.01.001 https://doaj.org/article/3d02a1101e5848baba3ee95a1fef1b3e |
Summary: | Objective: To investigate the presence of neutrophil extracellular traps (NETs) in vivo by analysing intestinal sections from experimentally Eimeria bovis- and naturally Eimeria arloingi-infected animals. Methods: Intestinal samples of Eimeria arloingi- and Eimeria bovis-infected animals were analysed by using immunohistochemical and fluorescence approach by using monoclonal antibodies. Results: Classical NET components were confirmed by co-localization of extracellular DNA being decorated with neutrophil elastase and histones in Eimeria-infected tissue samples. Here, extrusion of NETs was exclusively detected in intestinal polymorphonuclear neutrophils infiltrating Eimeria-infected sites. In vivo NETs were either found in close proximity or in direct contact to different Eimeria stages suggesting a stage-independent process. NETs were also found within the gut lumen driven by polymorphonuclear neutrophils that were contacting released oocysts. Conclusions: We postulate that NETs might play an important role in innate defence reactions in coccidiosis therefore significantly altering the outcome of infection. |
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