Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B

• Main Authors: László Gubicza, Erika Fehér, Katalin Bélafi-Bakó, Nándor Nemestóthy
• Format: Article in Journal/Newspaper
• Language: English
• Published: University of Zagreb Faculty of Food Technology and Biotechnology 2008
• Subjects: fusel oil; esterification; lipase; ping-pong bi-bi mechanism; Biotechnology; TP248.13-248.65; Food processing and manufacture; TP368-456; Antarc*; Antarctica
• Online Access: https://doaj.org/article/3c70d011893248db8be6b539cb625c56

Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a complex kinetic model. The parameters of the model were determined by numerical methods. It was found that four-parameter model fitted well with the experimental results and described properly the enzymatic reaction.