Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B

Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibiti...

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Bibliographic Details
Main Authors: László Gubicza, Erika Fehér, Katalin Bélafi-Bakó, Nándor Nemestóthy
Format: Article in Journal/Newspaper
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2008
Subjects:
fusel oil
esterification
lipase
ping-pong bi-bi mechanism
Biotechnology
TP248.13-248.65
Food processing and manufacture
TP368-456
Antarc*
Antarctica
Online Access:https://doaj.org/article/3c70d011893248db8be6b539cb625c56
Description
Summary:Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a complex kinetic model. The parameters of the model were determined by numerical methods. It was found that four-parameter model fitted well with the experimental results and described properly the enzymatic reaction.

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