Rapid Screening Alpha-Glucosidase Inhibitors from Polygoni Vivipari Rhizoma by Multi-Step Matrix Solid-Phase Dispersion, Ultrafiltration and HPLC

Polygoni Vivipari Rhizoma (PVR), the dried root of Polygonum viviparum , has been used as herbal medicine in China for a long time. In the present study, a new method based on multi-step matrix solid-phase dispersion (MSPD), ultrafiltration and high performance liquid chromatography (HPLC) for scree...

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Bibliographic Details
Published in:Molecules
Main Authors: Haoxiang Li, Zhuobin He, Qianhui Shen, Weifeng Fan, Guoying Tan, Yuansheng Zou, Quanxi Mei, Zhengming Qian
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2021
Subjects:
Polygoni Vivipari Rhizoma
alpha-glucosidase inhibitors
MSPD
ultrafiltration
HPLC
Organic chemistry
QD241-441
Polygonum viviparum
Online Access:https://doi.org/10.3390/molecules26206111
https://doaj.org/article/3ad9a94f310f40db8774ba221e391f62
Description
Summary:Polygoni Vivipari Rhizoma (PVR), the dried root of Polygonum viviparum , has been used as herbal medicine in China for a long time. In the present study, a new method based on multi-step matrix solid-phase dispersion (MSPD), ultrafiltration and high performance liquid chromatography (HPLC) for screening alpha-glucosidase inhibitors (AGIs) from PVR was proposed. First, three different PVR extractions were prepared by multi-step MSPD with 15% methanol, 60% methanol and 100% methanol. Second, the alpha-glucosidase inhibition tests for the three extracts were carried out, and the 60% methanol extraction showed the best activity. Then, the AGIs screening experiment was performed with ultrafiltration and HPLC analysis using the 60% methanol extraction. Seven binding components (quercetin−3− O −vicianoside, quercetin 3− O −neohesperidoside, rutin, hyperoside, quercetin 3− O −glucuronide, luteolin−7− O −neohesperidoside, kaempferol 3−glucuronide) were found. These seven components were further validated as the AGIs by molecular docking analysis. The developed method was a rapid and efficient tool for screening AGIs from PVR, which provided scientific data for the bioactive components study of PVR.

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