Growth and Survival of Escherichia albertii in Food and Environmental Water at Various Temperatures

• Published in: Journal of Food Protection
• Main Authors: Shouhei Hirose, Noriko Konishi, Mika Sato, Kyouhei Suzumura, Hiromi Obata, Kayoko Ohtsuka, Rie Doi, Keiichi Goto, Akemi Kai, Sakura Arai, Yukiko Hara-Kudo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2024
• Subjects: Environmental water; Escherichia albertii; Food; Growth; Survival; Temperature; Food processing and manufacture; TP368-456; Nutrition. Foods and food supply; TX341-641; Pacific oyster
• Online Access: https://doi.org/10.1016/j.jfp.2024.100249; https://doaj.org/article/39f7f4f1ffca4eb89920ff25ea2f234c

Escherichia albertii is an emerging foodborne pathogen that causes diarrhea. E. albertii has been isolated from various foods, including pork and chicken meat, and environmental waters, such as river water. Although many food poisoning cases have been reported, there have been insufficient analyses of bacterial population behaviors in food and environmental water. In this study, we inoculated 2–5 log CFU of E. albertii into 25 g of pork, chicken meat, Japanese rock oyster, Pacific oyster, and 300 mL of well water and seawater at 4°C, 10°C, 20°C, and 30°C, and analyzed the bacterial population behavior in food and environmental water. After 3 days at 4°C, the population of E. albertii strain EA21 and EA24 in foods maintained approximately 4 log CFU/25 g. After 3 days at 10°C, the population of E. albertii strains in pork and oysters maintained approximately 4 log CFU/25 g, and that in chicken meat increased to approximately 5–6 log CFU/25 g. After 2 days at 20°C, E. albertii strains grew to approximately 6–7 log CFU/25 g in pork and chicken meat, and E. albertii strain EA21 but not EA24 grew to 4.5 log CFU/25 g in Japanese rock oyster, E. albertii strain EA21 but not EA24 slightly grew to 3.1 log CFU/25 g in Pacific oyster. After 1 day at 30°C, E. albertii strains grew to approximately 7–8 log CFU/25 g in chicken meat and pork, grew to approximately 4–6 log CFU/25 g in Japanese rock oyster, and 6–7 log CFU/25 g in Pacific oyster. These results suggest that E. albertii survives without growth below 4°C and grew rapidly at 20°C and 30°C in foods, especially in meat. E. albertii strains did not grow in well water and seawater at 4°C, 10°C, 20°C, and 30°C. The population of E. albertii strains in well water and seawater decreased faster at 30°C than at 4°C, 10°C, and 20°C, suggesting that E. albertii has low viability at 30°C in environmental water.