Fecal specimens preparation methods for PCR diagnosis of human taeniosis

Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragm...

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Bibliographic Details
Main Authors: Nunes Cáris Maroni, Lima Luiz Gustavo Ferraz, Manoel Camila Santos, Pereira Rodrigo Norberto, Nakano Mauro Massaharu, Garcia José Fernando
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 2006
Subjects:
Taenia saginata
DNA extraction
Polymerase chain reaction (PCR)
Fecal specimens
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doaj.org/article/3911e36c9e274875ba0669b7121e8955
Description
Summary:Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®.

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