Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution

Candida antarctica lipase B (CALB) is a serine lipase. Here, the authors use directed evolution to exchange serine with cysteine in the catalytic triad of the enzyme, thereby obtaining a highly active CALB variant that — unlike the wild type — accommodates bulky substrates.

Bibliographic Details
Published in:Nature Communications
Main Authors: Yixin Cen, Warispreet Singh, Mamatjan Arkin, Thomas S. Moody, Meilan Huang, Jiahai Zhou, Qi Wu, Manfred T. Reetz
Format: Article in Journal/Newspaper
Language:English
Published: Nature Portfolio 2019
Subjects:
Science
Q
Antarc*
Antarctica
Online Access:https://doi.org/10.1038/s41467-019-11155-3
https://doaj.org/article/382d844422024dc1aad5ab0dfe4e992c
Description
Summary:Candida antarctica lipase B (CALB) is a serine lipase. Here, the authors use directed evolution to exchange serine with cysteine in the catalytic triad of the enzyme, thereby obtaining a highly active CALB variant that — unlike the wild type — accommodates bulky substrates.

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