RAPD-PCR – still a suitable Method for Genetically Underexplored Species?
Saithe (Pollachius virens) is a commercially important fish species; the annual catch quota in the Northeast Atlantic exceeds 100.000 t. Despite that saithe is underexplored from a fish population genetically view. Because saithe is a highly migratory species, which undergoes a long larval drift, th...
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ftdoajarticles:oai:doaj.org/article:36a0f7e1cc99468bb19ccc55a11deaf7 2023-05-15T17:35:14+02:00 RAPD-PCR – still a suitable Method for Genetically Underexplored Species? Konstanze Ursula Behrmann 2015-11-01T00:00:00Z https://doi.org/10.3389/conf.fmars.2015.03.00096 https://doaj.org/article/36a0f7e1cc99468bb19ccc55a11deaf7 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/conf.fmars.2015.03.00096/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/conf.fmars.2015.03.00096 https://doaj.org/article/36a0f7e1cc99468bb19ccc55a11deaf7 Frontiers in Marine Science, Vol 2 (2015) Migration Population Genetics fingerprinting RAPD-PCR Pollachius virens Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2015 ftdoajarticles https://doi.org/10.3389/conf.fmars.2015.03.00096 2022-12-31T04:39:51Z Saithe (Pollachius virens) is a commercially important fish species; the annual catch quota in the Northeast Atlantic exceeds 100.000 t. Despite that saithe is underexplored from a fish population genetically view. Because saithe is a highly migratory species, which undergoes a long larval drift, the population structure of saithe within the Northeast Atlantic is not fully understood. Models used as a basis for the management plan are based on tagging studies, which have been carried out in the 1960th. But still there are doubts regarding the numbers of stocks living in the Northeast Atlantic. Migration routes are affected by salmon farming, growing steadily from the 1990th. In the last years a hyperstability of the saithe stock in the North Sea had been detected underlining the need to have a closer look on the saithe stocks in the Northeast Atlantic. Random amplified polymorphic DNA (RAPD) - PCR is a DNA fingerprinting technique often used in species identification and population genetic research for species, whose genome has not been sequenced very extensive as being the case for most of the food fishes. We applied RAPD-PCR in a study of saithe populations from the North Atlantic. The suitability of RAPD-PCR was improved by optimisations for enhanced reproducibility. The “classical” protocol for RAPD-PCR was modified by increasing the annealing temperature and shortening the time of annealing, providing a much better reproducibility. Thus, RAPD-PCR was found to be a straightforward and low-cost way, compared to other population genetic tools, to get a first insight into the population structure of less sequenced fish species within a very short time, being useful for preliminary studies or laboratories without large capacities for DNA sequencing. Article in Journal/Newspaper North Atlantic Northeast Atlantic Directory of Open Access Journals: DOAJ Articles Frontiers in Marine Science 2 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Migration Population Genetics fingerprinting RAPD-PCR Pollachius virens Science Q General. Including nature conservation geographical distribution QH1-199.5 |
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Migration Population Genetics fingerprinting RAPD-PCR Pollachius virens Science Q General. Including nature conservation geographical distribution QH1-199.5 Konstanze Ursula Behrmann RAPD-PCR – still a suitable Method for Genetically Underexplored Species? |
topic_facet |
Migration Population Genetics fingerprinting RAPD-PCR Pollachius virens Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
Saithe (Pollachius virens) is a commercially important fish species; the annual catch quota in the Northeast Atlantic exceeds 100.000 t. Despite that saithe is underexplored from a fish population genetically view. Because saithe is a highly migratory species, which undergoes a long larval drift, the population structure of saithe within the Northeast Atlantic is not fully understood. Models used as a basis for the management plan are based on tagging studies, which have been carried out in the 1960th. But still there are doubts regarding the numbers of stocks living in the Northeast Atlantic. Migration routes are affected by salmon farming, growing steadily from the 1990th. In the last years a hyperstability of the saithe stock in the North Sea had been detected underlining the need to have a closer look on the saithe stocks in the Northeast Atlantic. Random amplified polymorphic DNA (RAPD) - PCR is a DNA fingerprinting technique often used in species identification and population genetic research for species, whose genome has not been sequenced very extensive as being the case for most of the food fishes. We applied RAPD-PCR in a study of saithe populations from the North Atlantic. The suitability of RAPD-PCR was improved by optimisations for enhanced reproducibility. The “classical” protocol for RAPD-PCR was modified by increasing the annealing temperature and shortening the time of annealing, providing a much better reproducibility. Thus, RAPD-PCR was found to be a straightforward and low-cost way, compared to other population genetic tools, to get a first insight into the population structure of less sequenced fish species within a very short time, being useful for preliminary studies or laboratories without large capacities for DNA sequencing. |
format |
Article in Journal/Newspaper |
author |
Konstanze Ursula Behrmann |
author_facet |
Konstanze Ursula Behrmann |
author_sort |
Konstanze Ursula Behrmann |
title |
RAPD-PCR – still a suitable Method for Genetically Underexplored Species? |
title_short |
RAPD-PCR – still a suitable Method for Genetically Underexplored Species? |
title_full |
RAPD-PCR – still a suitable Method for Genetically Underexplored Species? |
title_fullStr |
RAPD-PCR – still a suitable Method for Genetically Underexplored Species? |
title_full_unstemmed |
RAPD-PCR – still a suitable Method for Genetically Underexplored Species? |
title_sort |
rapd-pcr – still a suitable method for genetically underexplored species? |
publisher |
Frontiers Media S.A. |
publishDate |
2015 |
url |
https://doi.org/10.3389/conf.fmars.2015.03.00096 https://doaj.org/article/36a0f7e1cc99468bb19ccc55a11deaf7 |
genre |
North Atlantic Northeast Atlantic |
genre_facet |
North Atlantic Northeast Atlantic |
op_source |
Frontiers in Marine Science, Vol 2 (2015) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/conf.fmars.2015.03.00096/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/conf.fmars.2015.03.00096 https://doaj.org/article/36a0f7e1cc99468bb19ccc55a11deaf7 |
op_doi |
https://doi.org/10.3389/conf.fmars.2015.03.00096 |
container_title |
Frontiers in Marine Science |
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2 |
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1766134317505839104 |