Comparative omics directed gene discovery and rewiring for normal temperature-adaptive red pigment synthesis by polar psychrotrophic fungus Geomyces sp. WNF-15A

The Antarctic fungus Geomyces sp. WNF-15A can produce high-quality red pigments (AGRP) with good prospects for the use in food and cosmetic area. However, efficient AGRP synthesis relies on low-temperature and thus limits its industrial development. Here genome sequencing and comparative analysis we...

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Bibliographic Details
Published in:Synthetic and Systems Biotechnology
Main Authors: Haoyu Long, Jiawei Zhou, Yanna Ren, Jian Lu, Nengfei Wang, Haifeng Liu, Xiangshan Zhou, Menghao Cai
Format: Article in Journal/Newspaper
Language:English
Published: KeAi Communications Co., Ltd. 2024
Subjects:
Polar fungi
Red pigment
Cold-adaptive
Comparative omics
Food additives
Biotechnology
TP248.13-248.65
Biology (General)
QH301-705.5
Antarc*
Antarctic
Online Access:https://doi.org/10.1016/j.synbio.2024.07.002
https://doaj.org/article/35550dd703aa482e9f1bc214f0f6d33c
Description
Summary:The Antarctic fungus Geomyces sp. WNF-15A can produce high-quality red pigments (AGRP) with good prospects for the use in food and cosmetic area. However, efficient AGRP synthesis relies on low-temperature and thus limits its industrial development. Here genome sequencing and comparative analysis were performed on the wild-type versus to four mutants derived from natural mutagenesis and transposon insertion mutation. Eleven mutated genes were identified from 2309 SNPs and 256 Indels. A CRISPR-Cas9 gene-editing system was established for functional analysis of these genes. Deficiency of scaffold1.t692 and scaffold2.t704 with unknown functions highly improved AGRP synthesis at all tested temperatures. Of note, the two mutants produced comparable levels of AGRP at 20 °C to the wild-type at 14 °C. They also broke the normal-temperature limitation and effectively synthesized AGRP at 25 °C. Comparative metabolomic analysis revealed that deficiency of scaffold1.t692 improved AGRP synthesis by regulation of global metabolic pathways especially downregulation of the competitive pathways. Knockout of key genes responsible for the differential metabolites confirmed the metabolomic results. This study shows new clues for cold-adaptive regulatory mechanism of polar fungi. It also provides references for exploitation and utilization of psychrotrophic fungal resources.

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