Potential infectivity of blood from HBsAG asymptomatic carriers due to the presence of HBV-DNA and comparison with other markers of hbv infection Potencial de infectividade em sangue de portadores assintomáticos de HBsAg caracterizado pela presença de HBV-DNA e comparação com outros marcadores da Hepatite B.

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: M. Valeria Tedeschi, Cláudia Figueiredo Padilla, Iná Ferraz De Camargo, Clara F. Tachibana Yoshida
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1989
• Subjects: HBV-DNA; HBsAg; IgM anti-HBc Asymptomatic carriers; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46651989000600003; https://doaj.org/article/312489b71c8c4f3e93c92fdae0a1fd59

Serum samples from 356 HBsAg positive asymptomatic carriers, which were titrated by reverse passive hemagglutination, were analysed for the presence of HBV-DNA, HBsAg and IgM anti-HBc. The samples were divided in three classes, according to the titers of HBsAg and IgM anti-HBc and the distribution of HBV-DNA and HBsAg among these classes was studied. In the high titer class of HBsAg, 65% of samples have one or both markers against only 19% in the low titer class. From the total of 356 samples, 121 gave positive results for IgM anti-HBc (33.9%). From these, 38.9% of HBV-DNA and 47.9% of HBeAg were observed, whereas in samples with absence of IgM anti-HBc, 18.3% and 16.6% were respectively found. A higher frequency of agreement between all these markers was found in the class of high titers of HBsAg; however, HBV-DNA was detected in the low titer class of HBsAg and little or no IgM anti-HBc, showing potential blood infectivity even in HBsAg positive borderline samples. Em 356 soros HBsAg positivos de portadores assintomáticos, titulados por hemaglutinação passiva reversa, foi analisada a presença de HBV DNA, HBeAg e IgM anti-HBc, da seguinte forma: as amostras foram divididas em três classes de acordo com o título de HBsAg e IgM anti-HBc e foi verificada a distribuição de HBV DNA e HBsAg nestas classes. Em amostras com títulos elevados de HBsAg, 65% das amostras tiveram um ou ambos os marcadores HBV-DNA e HBsAg e as mesmas foram encontradas em apenas 19% das amostras com baixo título de HBsAg. Do total de 356 amostras, 121 foram positivas para IgM anti-HBc (33.9%). Destas, 38.9% de HBV-DNA e 47.9% de HBeAg foram observadas, ao passo que em amostras com ausência de IgM anti-HBc, 18.3% e 16.6% foram respectivamente encontrados. A concordância entre os três marcadores foi encontrada em. amostras com alto título de HBsAg, entretanto HBV-DNA foi também detectado em grupo de baixo título de HBsAg na ausência de IgM anti-HBc ou em níveis baixos, mostrando o potencial de infectividade mesmo em amostras de HBsAg com ...