Effects of chilled storage and pH of activating solution on different motility parameters in burbot (Lota lota) sperm

The effects of a simple saline solution prepared using two different pH (4.4 and 8.5) on sperm motility in burbot were investigated. Results were recorded during a 96-hour chilled storage (4°C) in 24-hour intervals. Measurements were focused on the detailed characteristics of motility using 12 param...

Full description

Bibliographic Details
Published in:Czech Journal of Animal Science
Main Authors: Zoltán Bokor, Balázs Csorbai, Levente Várkonyi, Zsolt Szári, Ferenc Fodor, István Ittzés, Daniel Żarski, Béla Urbányi, Gergely Bernáth
Format: Article in Journal/Newspaper
Language:English
Published: Czech Academy of Agricultural Sciences 2018
Subjects:
short-term storage
activation
lota lota
sperm analysis
casa parameters
Animal culture
SF1-1100
Burbot
Lota lota
lota
Online Access:https://doi.org/10.17221/122/2017-CJAS
https://doaj.org/article/2dbb2f90c4b74657af5b7b7606f7eb26
Description
Summary:The effects of a simple saline solution prepared using two different pH (4.4 and 8.5) on sperm motility in burbot were investigated. Results were recorded during a 96-hour chilled storage (4°C) in 24-hour intervals. Measurements were focused on the detailed characteristics of motility using 12 parameters obtained from the Computer-assisted Sperm Analysis (CASA). Significantly higher progressive motility (pMOT), distance average path (DAP), distance curved line, distance straight line (DSL), average path velocity (VAP), curvilinear velocity, straight line velocity, and beat cross frequency (BCF) were observed with the activating solution buffered at pH 8.5 in comparison with pH 4.4. Already after 24 h a significant reduction was measured in pMOT (0 h: 49 ± 24%, 24 h: 12 ± 7%). Similar decreasing tendency was recorded only after 72 h in DAP (0 h: 26 ± 4 µm/s, 72 h: 19 ± 9 µm/s), DSL (0 h: 21 ± 5 µm/s, 72 h: 17 ± 8 µm/s), VAP (0 h: 59 ± 9 µm/s, 72 h: 43 ± 21 µm/s), and BCF (0 h: 28 ± 2 Hz, 72 h: 18 ± 10 Hz). The response of different investigated CASA parameters to different treatments varied in our experiments. According to our studies, numerous burbot sperm motility parameters are sensitive to chilled storage and to low pH of the activating solution. Our results could support the effective sperm quality assessment and successful artificial propagation process in burbot.

Similar Items