Lipase Immobilized on MCFs as Biocatalysts for Kinetic and Dynamic Kinetic Resolution of sec -Alcohols

• Published in: Catalysts
• Main Authors: Dominika Stradomska, Monika Heba, Aleksandra Czernek, Nikodem Kuźnik, Danuta Gillner, Katarzyna Maresz, Wojciech Pudło, Andrzej Jarzębski, Katarzyna Szymańska
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2021
• Subjects: chiral secondary alcohols; resolution of enantiomers; dynamic kinetic resolution; DKR; synthesis of enantiomers; bicatalytic tandem; Chemical technology; TP1-1185; Chemistry; QD1-999; Antarc*; Antarctica
• Online Access: https://doi.org/10.3390/catal11040518; https://doaj.org/article/2b9b5b1d53a441bda16d0668d5a308fc

Dynamic kinetic resolution (DKR) is one of the most attractive methods for enantioselective synthesis. In the reported studies, lipase B from Candida antarctica (CALB) immobilized on siliceous mesoporous cellular foams (MCF) functionalized with different hydrophobic groups, and two ruthenium complexes with substituted cyclopentadienyl ligands were investigated as catalysts for the chemoenzymatic DKR of ( rac )-1-phenylethanol, using Novozym 435 as a benchmark biocatalyst. Studies on the ( rac )-1-phenylethanol transesterification reaction showed that CALB supported on MCFs grafted with methyl groups is a promising biocatalyst and isopropenyl acetate is a preferable acylation agent. Both Ru-complexes activated by K 3 PO 4 or t- BuOK, proved to be effective catalysts of the racemization reaction. The final DKR experiments using all catalysts combinations singled out, gave 96% conversion, and ( R )-1-phenylethyl acetate enantiomeric excess of 98% in 8 h using K 3 PO 4 activator.