Evaluation of phenotypic markers associated with pathogenicity in the genus Listeria Avaliação de marcadores fenotípicos associados à patogenicidade no gênero Listeria

A total of 130 Listeria strains were tested in order to evaluate lecithinase production and capacity for Congo red adsorption as markers of pathogenicity. The strains were identified according to acid production from sugars and by the CAMP test and the data were correlated with the ability to produc...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Zilma das Graças Nunes, Ernesto Hofer
Format: Article in Journal/Newspaper
Language:English
Portuguese
Published: Universidade de São Paulo 1994
Subjects:
Online Access:https://doi.org/10.1590/S0036-46651994000400001
https://doaj.org/article/27bdb7874725473b8a4ac70a43f74906
Description
Summary:A total of 130 Listeria strains were tested in order to evaluate lecithinase production and capacity for Congo red adsorption as markers of pathogenicity. The strains were identified according to acid production from sugars and by the CAMP test and the data were correlated with the ability to produce keratoconjunctivitis in guinea pigs. L. monocytogenes cultures presented 51.8% and 88.8% positivity rates for Congo red adsorption and lecithinase production, respectively, whereas 80.8% and 100% for L. innocua cultures were negative for the two test, respectively Com propósito de avaliar a produção de lecitinase e a capacidade de adsorção do corante vermelho Congo como marcadores de patogenicidade, foram estudadas 130 amostras de Listeria. Estas amostras foram identificadas segundo a produção de ácido a partir de açúcares aliada ao teste CAMP, correlacionando-se estes dados à capacidade de produção de ceratoconjuntivite em cobaio. As culturas de L. monocytogenes apresentaram taxas de positividade para a adsorção do corante e produção de lecitinase de 51,8 e 88,8%, respectivamente, enquanto 80,8% e 100% das culturas de L. innocua foram negativas para os referidos testes.