Multilocus enzyme electrophoresis study of Bacillus sphaericus

Multilocus enzyme electrophoresis (MLEE) has been used in the study of some Bacillus species. In this work we applied MLEE and numerical analysis in the study of the Bacillus sphaericus group. B. sphaericus can be distinguished from other entomopathogenic Bacillus by a unique allele (NP-4). Within t...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz
Main Authors: Viviane Zahner, Hooman Momen
Format: Article in Journal/Newspaper
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 1995
Subjects:
Bacillus sphaericus
isoenzyme
taxonomy
Arctic medicine. Tropical medicine
RC955-962
Microbiology
QR1-502
Arctic
Online Access:https://doi.org/10.1590/S0074-02761995000100015
https://doaj.org/article/27032ca7d4314e3684df0653037abe0f
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Summary:Multilocus enzyme electrophoresis (MLEE) has been used in the study of some Bacillus species. In this work we applied MLEE and numerical analysis in the study of the Bacillus sphaericus group. B. sphaericus can be distinguished from other entomopathogenic Bacillus by a unique allele (NP-4). Within the species, all insect pathogens were recovered in the same phenetic cluster and all of these strains have the same band position (electrophoresis migration) on the agarose gel (ADH-2). The entomopathogenic group of B. sphaericus seems to be a clonal population, having two widespread frequent genotypes (zymovar 59 and zymovar 119).

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